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Antibodies > CHUK / IKKA / IKK Alpha > LS-C800875
 
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Catalog Number Size Price
LS-C800875-100 100 µl (1 mg/ml) $379 
LS-C800875-200 200 µl (1 mg/ml) $421 
CHUK / IKKA / IKK Alpha Antibody - 1:200 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHUK / IKKA / IKK Alpha Antibody - 1:50 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHUK / IKKA / IKK Alpha Antibody - 1:50 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHUK / IKKA / IKK Alpha Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CHUK / IKKA / IKK Alpha Antibody - Western blot analysis of IKK-alpha/IKK beta phosphorylation expression in TNF treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CHUK / IKKA / IKK Alpha Antibody - Western blot analysis of TIMP4 expression in NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.Western blot analysis of extracts of various cellslines using Phospho-PDK1 (Ser241) antibody.
CHUK / IKKA / IKK Alpha Antibody - Western blot analysis of IKK-alpha/IKK beta phosphorylation expression in TNF treated HeLa whole cells lysates. The lane on the right is treated with the antigen-specific peptide.
CHUK / IKKA / IKK Alpha Antibody - 1:200 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHUK / IKKA / IKK Alpha Antibody - 1:50 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHUK / IKKA / IKK Alpha Antibody - 1:50 staining human breast carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CHUK / IKKA / IKK Alpha Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CHUK / IKKA / IKK Alpha Antibody - Western blot analysis of IKK-alpha/IKK beta phosphorylation expression in TNF treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CHUK / IKKA / IKK Alpha Antibody - Western blot analysis of TIMP4 expression in NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.Western blot analysis of extracts of various cellslines using Phospho-PDK1 (Ser241) antibody.
CHUK / IKKA / IKK Alpha Antibody - Western blot analysis of IKK-alpha/IKK beta phosphorylation expression in TNF treated HeLa whole cells lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human CHUK / IKKA / IKK Alpha Antibody (phospho‑Ser176,phospho‑Ser177, IHC, IF, WB) LS‑C800875

Polyclonal Rabbit anti‑Human CHUK / IKKA / IKK Alpha Antibody (phospho‑Ser176,phospho‑Ser177, IHC, IF, WB) LS‑C800875

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Antibody:
CHUK / IKKA / IKK Alpha Rabbit anti-Human Polyclonal (pSer176,pSer177) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800875-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CHUK / IKKA / IKK Alpha Rabbit anti-Human Polyclonal (pSer176,pSer177) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IKK Alpha antibody LS-C800875 is an unconjugated rabbit polyclonal antibody to IKK Alpha (CHUK / IKKA) (pSer176,pSer177) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human CHUK / IKKA / IKK Alpha
Synonyms
CHUK | I-kappa-B kinase alpha | I-kappa-B kinase-alpha | Ikb kinase alpha | IKK-A | IKBKA | IKK1 | NFKBIKA | I-kappa-B kinase 1 | IkappaB kinase | IKK Alpha | IKK-alpha | IKKA | Transcription factor 16 | IkappaBalpha Kinase | IkB kinase alpha subunit | IKK-a kinase | Ikkalpha | TCF-16 | TCF16
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human IKK- alpha /IKK- beta around the phosphorylation site of Serine 177.
Epitope
pSer176,pSer177
Specificity
Phospho-IKK- alpha (Ser176) /IKK- beta (Ser177) Antibody detects endogenous levels of IKK- alpha /IKK- beta only when phosphorylated at Serine 177.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHUK / IKKA / IKK Alpha
O15111 NM_001278 NP_001269.3

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CHUK / IKKA / IKK Alpha Antibody - Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml]
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CHUK / IKKA / IKK Alpha Antibody - Anti-CHUK / IKK-Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ CHUK / IKKA / IKK Alpha Antibody (C‑Terminus) LS‑B355
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Applications: IHC, IHC - Paraffin, Western blot, ELISA
CHUK / IKKA / IKK Alpha Antibody - Anti-CHUK / IKK-Alpha antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:250.
IHC‑plus™ CHUK / IKKA / IKK Alpha Antibody (C‑Terminus) LS‑B2900
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Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
CHUK / IKKA / IKK Alpha Antibody - Immunohistochemical analysis of IKK alpha (pT23) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CHUK / IKKA / IKK Alpha Antibody (phospho‑Thr23) LS‑C352022
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, Western blot
See all CHUK / IKKA / IKK Alpha Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

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