Human CHUK / IKKA / IKK Alpha

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

IKKa peptide corresponding to the highly conserved C-Terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

C-Terminus

Recognizes human total IKK alpha protein at 85kD. Species Crossreactivity: mouse and rat.

• IHC
• IHC - Paraffin (1:250)
• Western blot (1:500 - 1:1000)
• Immunoprecipitation
• ELISA

Immunohistochemistry: LS-B2900 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2900 was determined to be 1:250. Western Blot: Detects a band at ~85kD.

0.1% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15111 NCBI: NM_001278 NP_001269.3