Target
Human CHUK / IKKA / IKK Alpha
Synonyms
CHUK | I-kappa-B kinase alpha | I-kappa-B kinase-alpha | Ikb kinase alpha | IKK-A | IKBKA | IKK1 | NFKBIKA | I-kappa-B kinase 1 | IkappaB kinase | IKK Alpha | IKK-alpha | IKKA | Transcription factor 16 | IkappaBalpha Kinase | IkB kinase alpha subunit | IKK-a kinase | Ikkalpha | TCF-16 | TCF16
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Immunogen
IKK a peptide corresponding to the highly conserved C-terminus region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B355 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B355 was determined to be 1:500.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHUK / IKKA / IKK Alpha