Target
Pig CELA1 / Pancreatic Elastase 1
Synonyms
CELA1 | El1a | Elastase I | ELA1 | Elastase 1, pancreatic | Elastase-1 | Pancreatic elastase | Pancreatic elastase I | Porcine pancreatic elastase | Pancreatic elastase 1 | Elastase
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Elastase [Porcine Pancreas].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity against Elastase from other tissues and species may occur but have not been specifically determined.
Applications
- IHC
- Western blot (1:500 - 1:2000)
- ELISA (1:5000 - 1:20000)
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Anti-Elastase has been assayed against 1.0 ug of Elastase [Porcine Pancreas] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:200 to 1:1000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CELA1 / Pancreatic Elastase 1