Target
Pig CELA1 / Pancreatic Elastase 1
Synonyms
CELA1 | El1a | Elastase I | ELA1 | Elastase 1, pancreatic | Elastase-1 | Pancreatic elastase | Pancreatic elastase I | Porcine pancreatic elastase | Pancreatic elastase 1 | Elastase
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Elastase [Porcine Pancreas].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity was reported for mouse Elastase (mouse embryonic pancreas). Cross reactivity against Elastase from other tissues and species has not been determined.
Applications
- Immunofluorescence (1:100)
- Western blot (1:500 - 1:3000)
- ELISA (1:500 - 1:3000)
Usage
Suitable for immunoblotting (western or dot blot), ELISA. This product has been assayed against 1.0 ug of Elastase [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no.
LS-C60884 and ABTS as a substrate for 30 minutes at room temperature.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CELA1 / Pancreatic Elastase 1