Rat CD71 / Transferrin Receptor

Mouse

Rat (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

OX-26

Unconjugated

Protein G purified

Low Endotoxin

PHA activated rat lymphocytes

Recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues. The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialized proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis. An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer's€™s, Parkinson's, Huntington's and Multiple Sclerosis. Is reported as suitable for use in immunoelectron microscopy. OX-26 detects a band of ~95kD in Western blotting under reducing conditions and ~195kD under non-reducing conditions reflecting it's homodimeric structure.

• IHC - Paraffin
• IHC - Frozen
• Immunofluorescence
• Western blot
• Flow Cytometry (1:10 - 1:100)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.

PBS

Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02786 NCBI: NM_003234 NP_003225.2