Mouse CD71 / Transferrin Receptor

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

8D3

Unconjugated

Protein G purified

Low Endotoxin. Also available Unmodified.

Mouse transformed endothelioma cell line t. end1.

Recognizes mouse CD71, a 95kD cell surface protein that is otherwise known as transferrin receptor. CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system. Recognizes native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse.

• IHC
• IHC - Frozen
• Flow Cytometry (1:100 - 1:200)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 Method sheets are available on request.

PBS

Store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02786 NCBI: NM_003234 NP_003225.2