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Catalog Number Size Price
LS-C800987-100 100 µl (1 mg/ml) $379 
LS-C800987-200 200 µl (1 mg/ml) $421 
CD45 / LCA Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CD45 / LCA Antibody - Western blot analysis of CD45 phosphorylation expression in TNF treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CD45 / LCA Antibody - Western blot analysis of Phospho-CD45 (Ser1007) antibody expression in TNF treated HeLa cells lysates. The lane on the right is treated with the antigen-specific peptide.
CD45 / LCA Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CD45 / LCA Antibody - Western blot analysis of CD45 phosphorylation expression in TNF treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CD45 / LCA Antibody - Western blot analysis of Phospho-CD45 (Ser1007) antibody expression in TNF treated HeLa cells lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human CD45 / LCA Antibody (phospho‑Ser1007, IF, WB) LS‑C800987

Polyclonal Rabbit anti‑Human CD45 / LCA Antibody (phospho‑Ser1007, IF, WB) LS‑C800987

Antibody:
CD45 / LCA Rabbit anti-Human Polyclonal (pSer1007) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800987-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD45 / LCA Rabbit anti-Human Polyclonal (pSer1007) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
LCA antibody LS-C800987 is an unconjugated rabbit polyclonal antibody to LCA (CD45) (pSer1007) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human CD45 / LCA
Synonyms
PTPRC | B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human CD45 around the phosphorylation site of Serine 1007.
Epitope
pSer1007
Specificity
Phospho-CD45 (Ser1007) Antibody detects endogenous levels of CD45 only when phosphorylated at Serine 1007.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD45 / LCA
P08575 NM_002838 NP_002829.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024