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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2941-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human CD45 / LCA Antibody (clone 3G4, aa1029‑1249, IHC, WB) LS‑B2941
IHC‑plus™ Monoclonal Mouse anti‑Human CD45 / LCA Antibody (clone 3G4, aa1029‑1249, IHC, WB) LS‑B2941
Note: This antibody replaces LS-C93717
Antibody:
CD45 / LCA Mouse anti-Human Monoclonal (aa1029-1249) (3G4) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CD45 / LCA Mouse anti-Human Monoclonal (aa1029-1249) (3G4) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
LCA antibody LS-B2941 is an unconjugated mouse monoclonal antibody to human LCA (CD45) (aa1029-1249). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CD45 / LCA
Synonyms
PTPRC | B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
3G4
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human CD45 (1029-1249aa) purified from E. coli.
Epitope
aa1029-1249
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000 - 1:3000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2941 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2941 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD45 / LCA
Validation
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CD45 / LCA Antibody (aa1029-1249, clone 3G4) for IHC, WB/Western, ELISA LS-B2941 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
