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Catalog Number Size Price
LS-C407997-10 10 µg $318 
LS-C407997-100 100 µg $470 
CD45 / LCA Antibody - CD45 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CD45 / LCA Antibody - IF analysis of CD68/CD45 using anti-CD68/CD45 antibody. CD68/CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL mouse anti-CD68 Antibody/rabbit anti CD45 Antibody overnight at 4°C. Cy3 conjugated Avidinand DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD45 / LCA Antibody - CD45 antibody Western blot. All lanes: Anti CD45 at 0.5 ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40 ug. Lane 2: HL-60 Whole Cell Lysate at 40 ug. Lane 3: K562 Whole Cell Lysate at 40 ug. Predicted band size: 220 kD. Observed band size: 220 kD.
CD45 / LCA Antibody - CD45 antibody IHC-paraffin. IHC(P): Human Tonsil Tissue.
CD45 / LCA Antibody - IF analysis of CD68/CD45 using anti-CD68/CD45 antibody. CD68/CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL mouse anti-CD68 Antibody/rabbit anti CD45 Antibody overnight at 4°C. Cy3 conjugated Avidinand DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD45 / LCA Antibody - CD45 antibody Western blot. All lanes: Anti CD45 at 0.5 ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40 ug. Lane 2: HL-60 Whole Cell Lysate at 40 ug. Lane 3: K562 Whole Cell Lysate at 40 ug. Predicted band size: 220 kD. Observed band size: 220 kD.
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Polyclonal Rabbit anti‑Human CD45 / LCA Antibody (aa1214‑1254, IHC, WB) LS‑C407997

Polyclonal Rabbit anti‑Human CD45 / LCA Antibody (aa1214‑1254, IHC, WB) LS‑C407997

Antibody:
CD45 / LCA Rabbit anti-Human Polyclonal (aa1214-1254) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407997-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD45 / LCA Rabbit anti-Human Polyclonal (aa1214-1254) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LCA antibody LS-C407997 is an unconjugated rabbit polyclonal antibody to human LCA (CD45) (aa1214-1254). Validated for IHC and WB.
Target
Human CD45 / LCA
Synonyms
PTPRC | B220 | CD45R | CD45 | gp180 | Leukocyte common antigen | L-CA | Leucocyte common antigen | LY5 | T200 | T200 glycoprotein | T200 leukocyte common antigen | CD45 antigen | LCA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human CD45(1214-1254 aa EQYQFLYDVIASTYPAQNGQVKKNNHQEDKIEFDNEVDKVK), different from the related mouse sequence by eight amino acids, and from the related rat sequence by ten amino acids.
Epitope
aa1214-1254
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD45 / LCA
P08575 NM_002838 NP_002829.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/7/2025