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Catalog Number Size Price
LS-C112557-0.1 0.1 mg (0.1 mg/ml) $365 
CD3E Antibody - Intracellular staining of JURKAT cells with anti-CD3 epsilon activation epitope (APA1/1) PE.

Monoclonal Mouse anti‑Human CD3E Antibody (clone APA1/1, PE, IHC, WB) LS‑C112557

Monoclonal Mouse anti‑Human CD3E Antibody (clone APA1/1, PE, IHC, WB) LS‑C112557

Antibody:
CD3E Mouse anti-Human Monoclonal (PE) (APA1/1) Antibody
Application:
IHC-Fr, ICC, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Phycoerythrin, Unmodified
Other formats:
Price
Catalog Number
$365
LS-C112557-0.1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD3E Mouse anti-Human Monoclonal (PE) (APA1/1) Antibody
Application:
IHC-Fr, ICC, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Phycoerythrin, Unmodified
Other formats:

Specifications

Description
CD3E antibody LS-C112557 is a PE-conjugated mouse monoclonal antibody to CD3E from human. It is reactive with human and mouse. Validated for Flow, ICC, IHC, IP and WB.
Target
Human CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
APA1/1
Conjugations
Phycoerythrin. Also available Unconjugated.
Purification
Size exclusion chromatography
Modifications
Unmodified
Immunogen
Purified human CD3 proteins isolated from thymus.
Specificity
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Applications
  • IHC - Frozen
  • ICC
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suggested working dilution is 5 microgram/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Presentation
PBS, 15mM sodium azide
Storage
Store at 2°C to 8°C. Do not freeze. Avoid prolonged exposure to light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD3E

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024