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Catalog Number Size Price
LS-C112447-0.1 0.1 mg (1 mg/ml) $316 
CD3E Antibody - Intracellular staining of human peripheral blood with anti-CD3 epsilon activation epitope (APA1/1) purified, GAM-FITC.

Monoclonal Mouse anti‑Human CD3E Antibody (clone APA1/1, IHC, WB) LS‑C112447

Monoclonal Mouse anti‑Human CD3E Antibody (clone APA1/1, IHC, WB) LS‑C112447

Antibody:
CD3E Mouse anti-Human Monoclonal (APA1/1) Antibody
Application:
IHC, IHC-Fr, ICC, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$316
LS-C112447-0.1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CD3E Mouse anti-Human Monoclonal (APA1/1) Antibody
Application:
IHC, IHC-Fr, ICC, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD3E antibody LS-C112447 is an unconjugated mouse monoclonal antibody to CD3E from human. It is reactive with human and mouse. Validated for Flow, ICC, IHC, IP and WB.
Target
Human CD3E
Synonyms
CD3E | CD3e antigen | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
APA1/1
Conjugations
Unconjugated. Also available conjugated with PE.
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Immunogen
Purified human CD3 proteins isolated from thymus.
Specificity
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD3E

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/26/2024