Target
Pig CD14
Synonyms
CD14 | CD14 molecule | CD14 antigen
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Phycoerythrin
Purification
Protein G purified
Immunogen
E. coli-derived recombinant porcine CD14.
Specificity
Recognizes porcine CD14. Mature porcine CD14 shares 70-75% aa identity with human, cow, sheep, water buffalo and horse CD14 and 61-62% aa identity with mouse and rat CD14.
Applications
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Western Blot: 1-2 ug/ml. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25 ug/ml. Add 10 ul to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. Direct ELISA: 0.5-1 ug/ml. The detection limit is ~1 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Lyophilized and resuspended forms: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee