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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3012-50 | 50 µg (0.5 mg/ml) | $485 |
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IHC‑plus™ Polyclonal Goat anti‑Human CD14 Antibody (aa71‑84, IHC, WB) LS‑B3012
IHC‑plus™ Polyclonal Goat anti‑Human CD14 Antibody (aa71‑84, IHC, WB) LS‑B3012
Note: This antibody replaces LS-C20140
Antibody:
CD14 Goat anti-Human Polyclonal (aa71-84) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
CD14 Goat anti-Human Polyclonal (aa71-84) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD14 antibody LS-B3012 is an unconjugated goat polyclonal antibody to human CD14 (aa71-84). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.
Epitope
aa71-84
Specificity
Human CD14.
Applications
- IHC
- IHC - Paraffin (4 µg/ml)
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3012 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3012 was determined to be 4 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD14
Validation
Anti-CD14 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.
Anti-CD14 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 4 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CD14 Antibody (aa71-84) for IHC, WB/Western LS-B3012 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Control of tumor-associated macrophages and T cells in glioblastoma via AHR and CD3910<sup> Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. fquintana@rics.bwh.harvard.edu. Maisa C Takenaka, Galina Gabriely, Veit Rothhammer, Ivan D Mascanfroni, Michael A Wheeler, Chun-Cheih Chao, Cristina Gutiérrez-Vázquez, Jessica Kenison, Emily C Tjon, Andreia Barroso, Tyler Vandeventer, Kalil Alves de Lima, Sonja Rothweiler, Lior Mayo, Soufiene Ghannam, Stephanie Zandee, Luke Healy, David Sherr, Mauricio F Farez , Alexandre Prat, Jack Antel, David A Reardon, Hailei Zhang, Simon C Robson, Gad Getz, Howard L Weiner, Francisco J Quintana. Nature neuroscience. 2019 May;;22:729-740.
P2Y12 receptor is expressed on human microglia under physiological conditions throughout development and is sensitive to neuroinflammatory diseases. Alexander Mildner, Hao Huang, Josefine Radke, Werner Stenzel, Josef Priller. Glia. 2017 Feburary;65:375-387.
Customer Reviews (0)
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Species:
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Applications:
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Species:
Human, Monkey, Bovine, Cat, Dog, Pig, Sheep
Applications:
Flow Cytometry
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Species:
Human, Monkey, Bovine, Cat, Dog, Goat, Mink, Pig, Rabbit, Sheep, Cynomolgus
Applications:
Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
