Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C661927-10 10 µg $318 
LS-C661927-100 100 µg $470 
CD14 Antibody - Immunohistochemistry - Anti-CD14 Picoband Antibody
CD14 Antibody - Immunohistochemistry - Anti-CD14 Picoband Antibody
CD14 Antibody - Immunohistochemistry - Anti-CD14 Picoband Antibody
CD14 Antibody - Western blot analysis of CD14 using anti-CD14 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates,Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD14 at approximately 50KD. The expected band size for CD14 is at 40KD.
CD14 Antibody - Western blot - Anti-CD14 Picoband Antibody
CD14 Antibody - Flow Cytometry analysis of mouse PBMC cells using anti-CD14 antibody. Overlay histogram showing mouse PBMC cells stained with anti-CD14 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD14 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CD14 Antibody - Immunohistochemistry - Anti-CD14 Picoband Antibody
CD14 Antibody - Immunohistochemistry - Anti-CD14 Picoband Antibody
CD14 Antibody - Immunohistochemistry - Anti-CD14 Picoband Antibody
CD14 Antibody - Western blot analysis of CD14 using anti-CD14 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates,Lane 2: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD14 at approximately 50KD. The expected band size for CD14 is at 40KD.
CD14 Antibody - Western blot - Anti-CD14 Picoband Antibody
CD14 Antibody - Flow Cytometry analysis of mouse PBMC cells using anti-CD14 antibody. Overlay histogram showing mouse PBMC cells stained with anti-CD14 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD14 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1 of 6
2 of 6
3 of 6
4 of 6
5 of 6
6 of 6

Monoclonal Mouse anti‑Mouse CD14 Antibody (IHC, WB) LS‑C661927

Monoclonal Mouse anti‑Mouse CD14 Antibody (IHC, WB) LS‑C661927

Antibody:
CD14 Mouse anti-Mouse Monoclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661927-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD14 Mouse anti-Mouse Monoclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
CD14 antibody LS-C661927 is an unconjugated mouse monoclonal antibody to mouse CD14. Validated for ELISA, IHC and WB.
Target
Mouse CD14
Synonyms
CD14 | CD14 molecule | CD14 antigen
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse CD14 recombinant protein (Position: S16-A260).
Specificity
Detected on peritoneal macrophages (at protein level) (PubMed:8612135). Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level) (PubMed:19362712).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD14
P08571 NM_000591 NP_000582.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/30/2024