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Catalog Number Size Price
LS-C209697-100 100 µg (1 mg/ml) $574 
BrdU Antibody - Immunofluorescence Microscopy of Mouse Anti-BrdU antibody. Tissue: OCT-embedded E10.5 mouse embryo. Localization: 40X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:500 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4 degrees C. Secondary antibody: Alexa Fluor 488 Anti-Mouse secondary antibody at 1:200 for 45 min at RT. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.

Monoclonal Mouse BrdU Antibody (clone 29G6.E8, IHC, IF, WB) LS‑C209697

Monoclonal Mouse BrdU Antibody (clone 29G6.E8, IHC, IF, WB) LS‑C209697

Antibody:
BrdU Mouse Monoclonal (29G6.E8) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C209697-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BrdU Mouse Monoclonal (29G6.E8) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
BrdU antibody LS-C209697 is an unconjugated mouse monoclonal antibody to BrdU. Validated for ELISA, Flow, IF, IHC and WB.
Host
Mouse
Clonality
IgG1,l Monoclonal
Clone
29G6.E8
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with Bromodeoxyuridine-KLH followed by hybridoma development.
Specificity
This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence (1:500 - 1:3000)
  • Western blot (1:2000 - 1:5000)
  • Flow Cytometry (1:50 - 1:100)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-BrdU Antibody has been tested as suitable for immunoblot assays. Antibody may be suitable for additional immunoassays including immunofluorescence, flow cytometry and immunohistochemistry. Antibody will detect incorporated BrdU thymidine analog from replicated cells.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/24/2024