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Catalog Number Size Price
LS-C745342-25 25 µl (1.1 mg/ml) $304 
BrdU Antibody - Immunofluorescence microscopy images of paraformaldehyde-fixed, paraffin-embedded pancreas sections stained with antibodies against BrdU (red or pink) and counterstained with DAPI (blue) and imaged with a 40× objective. DAPI stained nuclei (blue) indicate non-dividing cells, immunostained red and pink nuclei indicate actively dividing pancreatic ß-cells. The antibodies were diluted to 2.7 µg/ml. and incubated with tissue sections overnight at 4 degrees. Donkey anti-rabbit secondary antibody was diluted 1:2500.
BrdU Antibody - Immunofluorescence Microscopy of rabbit anti-BrdU antibody. Tissue: 293T cells transfected expressing GFP-MCM9 L. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Anti-rabbit ATTO550 secondary antibody at 1:10,000 for 45 min at RT. Localization: BrdU is nuclear. Staining: BrdU in merged image shows with green and purple fluorescent signal with DAPI nuclear counterstain.
BrdU Antibody - Bromodeoxyuridine (BrdU) chemical structure representation.
BrdU Antibody - Immunofluorescence microscopy images of paraformaldehyde-fixed, paraffin-embedded pancreas sections stained with antibodies against BrdU (red or pink) and counterstained with DAPI (blue) and imaged with a 40× objective. DAPI stained nuclei (blue) indicate non-dividing cells, immunostained red and pink nuclei indicate actively dividing pancreatic ß-cells. The antibodies were diluted to 2.7 µg/ml. and incubated with tissue sections overnight at 4 degrees. Donkey anti-rabbit secondary antibody was diluted 1:2500.
BrdU Antibody - Immunofluorescence Microscopy of rabbit anti-BrdU antibody. Tissue: 293T cells transfected expressing GFP-MCM9 L. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Anti-rabbit ATTO550 secondary antibody at 1:10,000 for 45 min at RT. Localization: BrdU is nuclear. Staining: BrdU in merged image shows with green and purple fluorescent signal with DAPI nuclear counterstain.
BrdU Antibody - Bromodeoxyuridine (BrdU) chemical structure representation.
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Polyclonal Rabbit BrdU Antibody (IHC, IF, WB) LS‑C745342

Polyclonal Rabbit BrdU Antibody (IHC, IF, WB) LS‑C745342

Antibody:
BrdU Rabbit Polyclonal Antibody
Application:
IHC, IF, WB, IP, Flo, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C745342-25
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BrdU Rabbit Polyclonal Antibody
Application:
IHC, IF, WB, IP, Flo, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
BrdU antibody LS-C745342 is an unconjugated rabbit polyclonal antibody to BrdU. Validated for ELISA, Flow, IF, IHC, IP and WB.
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with Bromodeoxyuridine-KLH.
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence (1:500)
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:50 - 1:100)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/22/2024