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Catalog Number	Size	Price
LS-C358422-100	100 µl (1 mg/ml)	$315

AURKA / Aurora-A Antibody - Immunohistochemical analysis of Aurora A (pT288) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

AURKA / Aurora-A Antibody - Immunofluorescent analysis of Aurora A (pT288) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

AURKA / Aurora-A Antibody - Western blot analysis of Aurora A (pT288) expression in DLD (A); mouse brain (B); rat brain (C) whole cell lysates.

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Polyclonal Rabbit anti‑Human AURKA / Aurora‑A Antibody (phospho‑Thr288, IHC, IF, WB) LS‑C358422

Name: Anti-AURKA / Aurora-A Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C358422-100
Price: 315.0000 USD
Availability: InStock

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Antibody:

AURKA / Aurora-A Rabbit anti-Human Polyclonal (pThr288) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Monkey, Mouse, Rat, Bovine, Pig, Sheep

Format:

Unconjugated, Unmodified

$315

LS-C358422-100

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(800) 227-6666

For Research Use Only

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Overview

Antibody:

AURKA / Aurora-A Rabbit anti-Human Polyclonal (pThr288) Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Monkey, Mouse, Rat, Bovine, Pig, Sheep

Format:

Unconjugated, Unmodified

Specifications

Description

Aurora-A antibody LS-C358422 is an unconjugated rabbit polyclonal antibody to Aurora-A (AURKA) (pThr288) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.

Target

Human AURKA / Aurora-A

Synonyms

See All AURKA / Aurora-A Antibodies

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat, Bovine, Pig, Sheep (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Aurora A (pT288).

Epitope

pThr288

Specificity

Recognizes endogenous levels of Aurora A (pT288) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About AURKA / Aurora-A

Uniprot: O14965 NCBI: NM_003600 NP_003591.2