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| Catalog Number | Size | Price |
|---|---|---|
| LS-B191-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Polyclonal Goat anti‑Human AURKA / Aurora‑A Antibody (aa394‑403, IHC, WB) LS‑B191
IHC‑plus™ Polyclonal Goat anti‑Human AURKA / Aurora‑A Antibody (aa394‑403, IHC, WB) LS‑B191
Note: This antibody replaces LS-C20129, LS-C126935
Antibody:
AURKA / Aurora-A Goat anti-Human Polyclonal (aa394-403) Antibody
Application:
IHC, IHC-P, WB, IP, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
AURKA / Aurora-A Goat anti-Human Polyclonal (aa394-403) Antibody
Application:
IHC, IHC-P, WB, IP, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Aurora-A antibody LS-B191 is an unconjugated goat polyclonal antibody to human Aurora-A (AURKA) (aa394-403). Validated for IHC, IP, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AURKA / Aurora-A
Synonyms
AURKA | AIK | AIRK1 | ARK1 | Aurora-A kinase | Aurora kinase A | Aurora/IPL1-like kinase | AYK1 | AURA | AURORA2 | Breast tumor-amplified kinase | Breast-tumor-amplified kinase | BTAK | HARK1 | IPL1-related kinase | Serine/threonine kinase 6 | STK7 | STK6 | PPP1R47 | ARK-1 | Aurora 2 | Aurora-A | Aurora-related kinase 1 | Aurora/IPL1-related kinase 1 | IAK1 | Serine/threonine kinase 15 | STK15
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-QNKESASKQS, from the C-Terminus of protein sequence according to NP_003591.2.
Epitope
aa394-403
Specificity
Human AURKA / Aurora-A. Reported variants represent identical protein: NP_003591.2; NP_940835.1; NP_940836.1; NP_940837.1; NP_940838.1; NP_940839.1
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.1 - 0.3 µg/ml)
- Immunoprecipitation
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B191 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B191 was determined to be 10 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About AURKA / Aurora-A
Validation
Anti-AURKA / Aurora-A antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AURKA / Aurora-A antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ AURKA / Aurora-A Antibody (aa394-403) for IHC, WB/Western, IP LS-B191 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat, Pig
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
