Target
Human AURKA / Aurora-A
Synonyms
AURKA | AIK | AIRK1 | ARK1 | Aurora-A kinase | Aurora kinase A | Aurora/IPL1-like kinase | AYK1 | AURA | AURORA2 | Breast tumor-amplified kinase | Breast-tumor-amplified kinase | BTAK | HARK1 | IPL1-related kinase | Serine/threonine kinase 6 | STK7 | STK6 | PPP1R47 | ARK-1 | Aurora 2 | Aurora-A | Aurora-related kinase 1 | Aurora/IPL1-related kinase 1 | IAK1 | Serine/threonine kinase 15 | STK15
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QNKESASKQS, from the C-Terminus of protein sequence according to NP_003591.2.
Specificity
Human AURKA / Aurora-A. Reported variants represent identical protein: NP_003591.2; NP_940835.1; NP_940836.1; NP_940837.1; NP_940838.1; NP_940839.1
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.1 - 0.3 µg/ml)
- Immunoprecipitation
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
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Usage
Immunohistochemistry: LS-B191 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B191 was determined to be 10 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee