Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C662031-10 10 µg $318 
LS-C662031-100 100 µg $470 
ANGPT1 / Angiopoietin-1 Antibody - Western blot analysis of Angiopoietin-1 using anti-Angiopoietin-1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human ANGPT1 protein 1ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Angiopoietin-1 at approximately 70KD. The expected band size for Angiopoietin-1 is at 57KD.

Monoclonal Mouse anti‑Human ANGPT1 / Angiopoietin‑1 Antibody (WB) LS‑C662031

Monoclonal Mouse anti‑Human ANGPT1 / Angiopoietin‑1 Antibody (WB) LS‑C662031

Antibody:
ANGPT1 / Angiopoietin-1 Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662031-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ANGPT1 / Angiopoietin-1 Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Angiopoietin-1 antibody LS-C662031 is an unconjugated mouse monoclonal antibody to human Angiopoietin-1 (ANGPT1). Validated for ELISA and WB.
Target
Human ANGPT1 / Angiopoietin-1
Synonyms
ANGPT1 | Angiopoietin 1 | Angiopoietin-1 | AGPT | AGP1 | ANG-1 | ANG1 | KIAA0003
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin-1 (204-241 aa KHKEELDTLKEEKENLQGLVTRQTYIIQELEKQLNRAT), different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANGPT1 / Angiopoietin-1
Q15389 NM_001146 NP_001137.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024