Mouse ANGPT1 / Angiopoietin-1

Rabbit

Mouse, Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated

Unmodified

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

aa15-45

This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.

• IHC
• IHC - Paraffin (1:200)
• Western blot (1:500 - 1:2000)
• Immunoprecipitation
• ELISA (1:5000 - 1:25000)

Immunohistochemistry: LS-B62 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues and mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B62 was determined to be 1:200.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q15389 NCBI: NM_001146 NP_001137.2