Target
Human ALPL / Alkaline Phosphatase
Synonyms
ALPL | Alkaline phosphomonoesterase | AP-TNAP | Alkaline phosphatase liver | APTNAP | Glycerophosphatase | Tissue-nonspecific ALP | TNAP | HOPS | Liver alkaline phosphatase | TNSALP
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-ELQKLNTNVAKN, from the internal region (near N-Terminus) of the protein sequence according to NP_000469.3.
Specificity
This antibody is expected to recognize isoform 1 (NP_000469.3) only. The immunizing peptide represents the N terminus of the mature protein.
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 55kDa band observed in Human Kidney, Lung and Adrenal Gland lysates (calculated MW of 57.3kDa according to NP_000469.3). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALPL / Alkaline Phosphatase