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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3666-50 | 50 µg (1 mg/ml) | $575 |
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2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Human ALPL / Alkaline Phosphatase Antibody (IHC, WB) LS‑B3666
IHC‑plus™ Monoclonal Mouse anti‑Human ALPL / Alkaline Phosphatase Antibody (IHC, WB) LS‑B3666
Note: This antibody replaces LS-C17269
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Alkaline Phosphatase antibody LS-B3666 is an unconjugated mouse monoclonal antibody to Alkaline Phosphatase (ALPL) from human. It is reactive with human and bovine. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ALPL / Alkaline Phosphatase
Synonyms
ALPL | Alkaline phosphomonoesterase | AP-TNAP | Alkaline phosphatase liver | APTNAP | Glycerophosphatase | Tissue-nonspecific ALP | TNAP | HOPS | Liver alkaline phosphatase | TNSALP
Host
Mouse
Reactivity
Human, Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Purified alkaline phosphatase (native) from human bone.
Specificity
Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3666 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3666 was determined to be 10 ug/ml. Suitable for use in ELISA and Western Blot. Western Blot: Stains a band of bone AP at ~90kD.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ALPL / Alkaline Phosphatase
Validation
Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ALPL / Alkaline Phosphatase Antibody for IHC, WB/Western, ELISA LS-B3666 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Cat, Pig, Rabbit
Applications:
Immunofluorescence, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse
Applications:
Western blot, ELISA
Species:
Human
Applications:
Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Species:
Human, Mouse, Rat
Applications:
Immunoprecipitation
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/12/2024
Date Requested: 11/12/2024
