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Catalog Number Size Price
LS-C413481-100 100 µl (1 mg/ml) $267 
Alpha Tubulin Antibody - Immunohistochemical analysis of Alpha-tubulin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Alpha Tubulin Antibody - Immunofluorescent analysis of Alpha-tubulin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Alpha Tubulin Antibody - Western blot analysis of Alpha-tubulin expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.
Alpha Tubulin Antibody - Immunoprecipitation of Alpha-tubulin from 0.5mg mouse brain whole cell extract lysate; using Anti-Alpha-tubulin Antibody.
Alpha Tubulin Antibody - Immunohistochemical analysis of Alpha-tubulin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Alpha Tubulin Antibody - Immunofluorescent analysis of Alpha-tubulin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Alpha Tubulin Antibody - Western blot analysis of Alpha-tubulin expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.
Alpha Tubulin Antibody - Immunoprecipitation of Alpha-tubulin from 0.5mg mouse brain whole cell extract lysate; using Anti-Alpha-tubulin Antibody.
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Monoclonal Mouse anti‑Human Alpha Tubulin Antibody (IHC, IF, WB) LS‑C413481

Monoclonal Mouse anti‑Human Alpha Tubulin Antibody (IHC, IF, WB) LS‑C413481

Antibody:
Alpha Tubulin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$267
LS-C413481-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Alpha Tubulin Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha Tubulin antibody LS-C413481 is an unconjugated mouse monoclonal antibody to Alpha Tubulin from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB.
Target
Human Alpha Tubulin
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
Recombinant protein corresponding to human Alpha-tubulin.
Specificity
Recognizes endogenous levels of Alpha-tubulin protein.
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:500)
  • ICC (1:100 - 1:200)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:5000 - 1:10000)
  • Immunoprecipitation (1:100 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/23/2024