Yeast Alpha Tubulin

Rat

Yeast (tested or 100% immunogen sequence identity)

Nephrotoma suturalis

IgG2a Monoclonal

YL1/2

Horseradish Peroxidase

Protein G purified

Unmodified

Yeast tubulin.

Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

• IHC - Frozen
• Western blot (1:100 - 1:1000)
• ELISA

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.01% Thimerosal

Store at 4°C. Do not freeze. Store undiluted. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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