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Antibodies > Alpha Tubulin > LS-C368771
 
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Catalog Number Size Price
LS-C368771-30 30 µl (1 mg/ml) $239 
LS-C368771-100 100 µl (1 mg/ml) $267 
LS-C368771-200 200 µl (1 mg/ml) $302 
Alpha Tubulin Antibody - Immunohistochemical analysis of Alpha-tubulin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Alpha Tubulin Antibody - Immunofluorescent analysis of Alpha-tubulin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Alpha Tubulin Antibody - Western blot analysis of Alpha-tubulin expression in HeLa (A); A549 (B); MCF7 (C); NIH3T3 (D) whole cell lysates.
Alpha Tubulin Antibody - Immunohistochemical analysis of Alpha-tubulin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Alpha Tubulin Antibody - Immunofluorescent analysis of Alpha-tubulin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Alpha Tubulin Antibody - Western blot analysis of Alpha-tubulin expression in HeLa (A); A549 (B); MCF7 (C); NIH3T3 (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human Alpha Tubulin Antibody (C‑Terminus, IHC, IF, WB) LS‑C368771

Polyclonal Rabbit anti‑Human Alpha Tubulin Antibody (C‑Terminus, IHC, IF, WB) LS‑C368771

Submit Review LSBio Guarantee
Antibody:
Alpha Tubulin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$239
LS-C368771-30
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Alpha Tubulin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Pig, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha Tubulin antibody LS-C368771 is an unconjugated rabbit polyclonal antibody to Alpha Tubulin (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
Target
Human Alpha Tubulin
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Pig, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Alpha-tubulin.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of Alpha-tubulin protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Customer Reviews (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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For questions about orders:
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