Target
Human ALB / Serum Albumin
Synonyms
ALB | Albumin (32 AA) | Albumin (AA 34) | Albumin | Growth-inhibiting protein 20 | PRO1341 | PRO0883 | Serum albumin | PRO0903
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase.
Also available
Unconjugated or conjugated with
Biotin,
FITC.
Purification
Ion exchange chromatography
Specificity
Human ALB / Albumin. Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal human serum and purified human albumin. One characteristic precipitin line is obtained against pooled normal human serum using different antigen/antibody concentration ratio's. Precipitin lines against normal human serum and purified human albumin give a reaction of full identity. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Applications
- IHC
- ELISA
- Applications tested for the base form of this product only
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Usage
This product is intended for use in enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e. g. ELISA) to identify and measure albumin in human serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:2000 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Use 1 ml of sterile Distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALB / Serum Albumin