Human ALB / Serum Albumin

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Biotin. Also available Unconjugated or conjugated with FITC, HRP.

Ion exchange chromatography

Unmodified

Human albumin.

Human ALB / Albumin. The defined antibody specificity is directed to albumin as tested against human sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against human serum and the purified albumin. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has not been tested in detail.

• IHC
• ELISA
• Applications tested for the base form of this product only

This product is intended for use in immunocytochemical and immunohistochemical use for the detection of albumin at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; in non-isotopic assay methodology (e. g. ELISA) to measure albumin in human serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:5000 and 1:50000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2

Use 1 ml of sterile Distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02768 NCBI: NM_000477 NP_000468.1