Human ALB / Serum Albumin

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin

Affinity chromatography

Unmodified

Human Serum Albumin.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Western blot (1:1000 - 1:5000)
• ELISA (1:60000)

Immunohistochemistry: LS-B325 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B325 was determined to be 2.5 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 10 mg/ml PEG, 0.01% Sodium Azide

Reconstitute in 1 mL of deionized water.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02768 NCBI: NM_000477 NP_000468.1