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Catalog Number Size Price
LS-C745158-50 50 µg (1 mg/ml) $562 
AKT2 Antibody - Immunohistochemistry of Rat monoclonal anti AKT2 unconjugated Antibody in human breast carcinoma. Tissue: Human Breast Cancer. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. antibody: anti-AKT2 at 2ug/ml for 1.5 hour @ room temp. Secondary Ab: mouse anti-rat at 1:50 for 45” RT.
AKT2 Antibody - Western Blot of Rat Anti-AKT2 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT2 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT 2.
AKT2 Antibody - Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 unconjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.
AKT2 Antibody - Flow Cytometry of Rat anti-AKT2 antibody. Cells: Jurkat Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT2 antibody at 1.0 µg/mL for 20 min at 4°C.
AKT2 Antibody - Immunohistochemistry of Rat monoclonal anti AKT2 unconjugated Antibody in human breast carcinoma. Tissue: Human Breast Cancer. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. antibody: anti-AKT2 at 2ug/ml for 1.5 hour @ room temp. Secondary Ab: mouse anti-rat at 1:50 for 45” RT.
AKT2 Antibody - Western Blot of Rat Anti-AKT2 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT2 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT 2.
AKT2 Antibody - Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 unconjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.
AKT2 Antibody - Flow Cytometry of Rat anti-AKT2 antibody. Cells: Jurkat Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT2 antibody at 1.0 µg/mL for 20 min at 4°C.
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Monoclonal Rat anti‑Human AKT2 Antibody (clone 16G11.E8, RPE, Internal, IHC, IF, WB) LS‑C745158

Monoclonal Rat anti‑Human AKT2 Antibody (clone 16G11.E8, RPE, Internal, IHC, IF, WB) LS‑C745158

Antibody:
AKT2 Rat anti-Human Monoclonal (Internal) (RPE) (16G11.E8) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
R. Phycoerythrin, Unmodified
Price
Catalog Number
$562
LS-C745158-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AKT2 Rat anti-Human Monoclonal (Internal) (RPE) (16G11.E8) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
R. Phycoerythrin, Unmodified

Specifications

Description
AKT2 antibody LS-C745158 is an RPE-conjugated rat monoclonal antibody to human AKT2 (Internal). Validated for ELISA, Flow, IF, IHC and WB.
Target
Human AKT2
Synonyms
AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
16G11.E8
Conjugations
R. Phycoerythrin
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT2 Antibody was produced in rat by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Epitope
Internal
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl
Reconstitution
Reconstitute in 50µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT2
P31751 NM_001626 NP_001617.1

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Requested From: United States
Date Requested: 12/25/2024