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Catalog Number	Size	Price
LS-C98942-400	400 µl	$393

AKT2 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AKT2 Antibody - Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (S474). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AKT2 immunoreactivity is localized to Cytoplasm significantly.

AKT2 Antibody - Western blot of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).

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Polyclonal Rabbit anti‑Human AKT2 Antibody (Ser474, IHC, IF, WB) LS‑C98942

Name: Anti-AKT2 Antibody | Rabbit anti-Human Polyclonal IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C98942-400
Price: 393.0000 USD
Availability: InStock

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Antibody:

AKT2 Rabbit anti-Human Polyclonal (Ser474) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$393

LS-C98942-400

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For Research Use Only

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Overview

Antibody:

AKT2 Rabbit anti-Human Polyclonal (Ser474) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

AKT2 antibody LS-C98942 is an unconjugated rabbit polyclonal antibody to human AKT2 (Ser474). Validated for IF, IHC and WB.

Target

Human AKT2

Synonyms

See All AKT2 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Mouse, Rat (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

Ser474

Specificity

This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from human AKT2.

Applications

IHC
IHC - Paraffin (1:10 - 1:50)
Immunofluorescence (1:10 - 1:50)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About AKT2

Uniprot: P31751 NCBI: NM_001626 NP_001617.1