Target
Human AKT2
Synonyms
AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Immunogen
Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Applications
- IHC (20 µg/ml)
- Western blot (1:500 - 1:2000)
- Flow Cytometry
- ELISA (1:2000 - 1:10000)
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Usage
Anti-AKT2 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee