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Catalog Number	Size	Price
LS-C756635-10	10 µg	$318
LS-C756635-100	100 µg	$470

AKT2 Antibody - Western blot analysis of AKT2 using anti-AKT2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysate, Lane 2: human 293T whole cell lysate, Lane 3: human HELA whole cell lysate, Lane 4: human Caco-2 whole cell lysate, Lane 5: human K562 whole cell lysate, Lane 6: human HL-60 whole cell lysate, Lane 7: human PC-3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AKT2 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

Monoclonal Mouse anti‑Human AKT2 Antibody (WB) LS‑C756635

Name: Anti-AKT2 Antibody | Mouse anti-Human Monoclonal ICC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756635-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

AKT2 Mouse anti-Human Monoclonal Antibody

Application:

ICC, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C756635-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

AKT2 Mouse anti-Human Monoclonal Antibody

Application:

ICC, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

AKT2 antibody LS-C756635 is an unconjugated mouse monoclonal antibody to human AKT2. Validated for ICC and WB.

Target

Human AKT2

Synonyms

See All AKT2 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human AKT2(454-481 aa DRYDSLGLLELDQRTHFPQFSYSASIRE), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

Specificity

No cross reactivity with other proteins.

Applications