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Catalog Number Size Price
LS-C353900-100 100 µl (1 mg/ml) $299 
AIRE Antibody - Immunofluorescent analysis of AIRE staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
AIRE Antibody - Western blot analysis of AIRE expression in Jurkat (A); mouse lung (B); rat lung (C) whole cell lysates.
AIRE Antibody - Immunofluorescent analysis of AIRE staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
AIRE Antibody - Western blot analysis of AIRE expression in Jurkat (A); mouse lung (B); rat lung (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human AIRE Antibody (Internal, IF, WB) LS‑C353900

Polyclonal Rabbit anti‑Human AIRE Antibody (Internal, IF, WB) LS‑C353900

Antibody:
AIRE Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353900-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AIRE Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AIRE antibody LS-C353900 is an unconjugated rabbit polyclonal antibody to AIRE (Internal) from human. It is reactive with human, mouse and rat. Validated for ICC, IF and WB.
Target
Human AIRE
Synonyms
AIRE | APSI | AIRE1 | Autoimmune regulator | APECED | APS1 | APECED protein | PGA1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human AIRE.
Epitope
Internal
Specificity
Recognizes endogenous levels of AIRE protein.
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AIRE
O43918 NM_000383 NP_000374.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024