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Catalog Number	Size	Price
LS-B1740-50	50 µg (0.5 mg/ml)	$485

AIRE Antibody - Anti-AIRE antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

AIRE Antibody - AIRE antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

AIRE Antibody - AIRE antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

AIRE Antibody - AIRE antibody (1µg/ml) staining of nuclear Jurkat cell (A), (2ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

AIRE Antibody - AIRE antibody flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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IHC‑plus™ Polyclonal Goat anti‑Human AIRE Antibody (aa534‑545, IHC, IF, WB) LS‑B1740

Name: IHC-plus™ AIRE Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B1740-50
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

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Note: This antibody replaces LS-C47544, LS-C122627

Antibody:

AIRE Goat anti-Human Polyclonal (aa534-545) Antibody

Application:

IHC, IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$485

LS-B1740-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

AIRE Goat anti-Human Polyclonal (aa534-545) Antibody

Application:

IHC, IHC-P, IF, WB, Peptide-ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

AIRE antibody LS-B1740 is an unconjugated goat polyclonal antibody to human AIRE (aa534-545). Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human AIRE

Synonyms

See All AIRE Antibodies

Host

Goat

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-QSMARPAAPFPS, from the C-Terminus of protein sequence according to NP_000374.1.

Epitope

aa534-545

Specificity

Human AIRE. This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).

Applications

IHC
IHC - Paraffin (2.5 µg/ml)
Immunofluorescence (10 µg/ml)
Western blot (1 - 2 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B1740 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1740 was determined to be 2.5 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About AIRE

Uniprot: O43918 NCBI: NM_000383 NP_000374.1

Validation

Anti-AIRE antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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About LSBio Validation process