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Catalog Number Size Price
LS-C662452-10 10 µg $318 
LS-C662452-100 100 µg $470 
AIRE Antibody - Western blot - Anti-AIRE/Aire 1 Picoband Antibody
AIRE Antibody - Flow Cytometry analysis of THP-1 cells using anti-AIRE antibody. Overlay histogram showing THP-1 cells stained with anti-AIRE antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIRE Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
AIRE Antibody - Flow Cytometry analysis of HL-60 cells using anti-AIRE antibody. Overlay histogram showing HL-60 cells stained with anti-AIRE antibody. (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIRE Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
AIRE Antibody - Western blot - Anti-AIRE/Aire 1 Picoband Antibody
AIRE Antibody - Flow Cytometry analysis of THP-1 cells using anti-AIRE antibody. Overlay histogram showing THP-1 cells stained with anti-AIRE antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIRE Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
AIRE Antibody - Flow Cytometry analysis of HL-60 cells using anti-AIRE antibody. Overlay histogram showing HL-60 cells stained with anti-AIRE antibody. (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIRE Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human AIRE Antibody (IHC, WB) LS‑C662452

Polyclonal Rabbit anti‑Human AIRE Antibody (IHC, WB) LS‑C662452

Antibody:
AIRE Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662452-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
AIRE Rabbit anti-Human Polyclonal Antibody
Application:
IHC, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AIRE antibody LS-C662452 is an unconjugated rabbit polyclonal antibody to human AIRE. Validated for Flow, ICC, IHC and WB.
Target
Human AIRE
Synonyms
AIRE | APSI | AIRE1 | Autoimmune regulator | APECED | APS1 | APECED protein | PGA1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human AIRE recombinant protein (Position: M1-K120). Human AIRE shares 87.2% amino acid (aa) sequence identity with mouse AIRE.
Specificity
Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.
Applications
  • IHC
  • ICC
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AIRE
O43918 NM_000383 NP_000374.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/22/2024