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Catalog Number Size Price
LS-C820800-20 20 µl $278 
LS-C820800-100 100 µl $427 
ACTB / Beta Actin Antibody - Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: NIH3T3 Whole Cell Lysate. Lane C: RAW264.7 Whole Cell Lysate. Lane D: Mouse slpeen tissue lysate. Lane E: Mouse lung tissue lysate. Lane F: Mouse brain tissue lysate. Lane G: C6 whole cell lysate. Lane H: PC-12 whole cell lysate. Lane I: Rat liver tissue lysate. Lane J: Rat lung tissue lysate. Lane K: Rat brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary: HRP Conjugated Goat anti-Mouse IgG (H+L) , at 1:10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.
ACTB / Beta Actin Antibody - Anti-beta-Actin mouse monoclonal antibody at 1:10000, 1:50000, 1:100000, 1:200000 dilution. Lysates/proteins per lane: HepG2 (30 ug), Hela (5 ug), Raw246.7 (30 ug). Secondary: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.
ACTB / Beta Actin Antibody - Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.
ACTB / Beta Actin Antibody - ACTB was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 0.5 uL anti-ACTB mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-ACTB mouse monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.
ACTB / Beta Actin Antibody - Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: NIH3T3 Whole Cell Lysate. Lane C: RAW264.7 Whole Cell Lysate. Lane D: Mouse slpeen tissue lysate. Lane E: Mouse lung tissue lysate. Lane F: Mouse brain tissue lysate. Lane G: C6 whole cell lysate. Lane H: PC-12 whole cell lysate. Lane I: Rat liver tissue lysate. Lane J: Rat lung tissue lysate. Lane K: Rat brain tissue lysate. Lysates/proteins at 30 ug per lane. Secondary: HRP Conjugated Goat anti-Mouse IgG (H+L) , at 1:10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.
ACTB / Beta Actin Antibody - Anti-beta-Actin mouse monoclonal antibody at 1:10000, 1:50000, 1:100000, 1:200000 dilution. Lysates/proteins per lane: HepG2 (30 ug), Hela (5 ug), Raw246.7 (30 ug). Secondary: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.
ACTB / Beta Actin Antibody - Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 44 kDa.
ACTB / Beta Actin Antibody - ACTB was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 0.5 uL anti-ACTB mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-ACTB mouse monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.
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Monoclonal Mouse anti‑Human ACTB / Beta Actin Antibody (clone 10, Preservative Free, WB) LS‑C820800

Monoclonal Mouse anti‑Human ACTB / Beta Actin Antibody (clone 10, Preservative Free, WB) LS‑C820800

Antibody:
ACTB / Beta Actin Mouse anti-Human Monoclonal (Preservative Free) (10) Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$278
LS-C820800-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Mouse anti-Human Monoclonal (Preservative Free) (10) Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
Beta Actin antibody LS-C820800 is an unconjugated mouse monoclonal antibody to human Beta Actin (ACTB). Validated for IP and WB.
Target
Human ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
10
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the N-Terminus of Human beta Actin / ACTB
Specificity
Human Beta-Actin
Applications
  • Western blot (1:2500 - 1:200000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 7/22/2024