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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1

S. pyogenes Cas9

Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Target Details

Target Name: Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1

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Products
Antibodies (23)
Primary (23)
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 (23)
Human (5)
Staphylococcus aureus (3)
Streptococcus pyogenes (19)
IHC (1)
WB (22)
ELISA (10)
IF (12)
IP (6)
rabbit (9)
mouse (14)
IgG (7)
IgG1 (5)
IgG1,k (2)
IgG2a (2)
IgG2b (2)
IgG2b,k (3)
monoclonal mc (15)
polyclonal pc (8)
11C12 (1)
14B6 (1)
26H10 (1)
4A1 (1)
6F7 (1)
7A9-3A3 (1)
OTI1C8 (2)
OTI2B9 (2)
OTI5E5 (2)
OTI6D9 (2)
Biotin Conjugated (2)
Carrier-free (4)
HRP Conjugated (2)
Liquid (2)
Lyophilized (2)
Unconjugated (19)
aa1-1166 (8)
C-Terminus (6)
C-term (1)
aa1150-1200 (1)
No (23)
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (C-Terminus) Antibody
Streptococcus pyogenes
ELISA, IF, WB
Unconjugated
100 µg/$567
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCAS-Guide vector (GE100002, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAS9.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (aa1150-1200) Antibody
Streptococcus pyogenes
WB
Unconjugated
100 µl/$417
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Anti S. Pyogenes CRISPR/Cas9, clone 7A9
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (7A9-3A3) Antibody
Streptococcus pyogenes
IF, WB
Unconjugated
0.1 mg/$509
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (C-Terminus) (Lyophilized) (Biotin) Antibody
Streptococcus pyogenes
ELISA, IF, WB
Biotin Conjugated, Lyophilized
100 µg/$544
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (aa1-1166) (OTI2B9) Antibody
Streptococcus pyogenes
WB
Unconjugated
100 µl/$434
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody at 1:5000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal Antibody
Streptococcus pyogenes
WB
Unconjugated
20 µl/$275; 100 µl/$389
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - High Sensitivity: GenCrispr Cas9 antibody can detect as low as 0.5 ng GenCrispr Cas9 nuclease. GenCrispr Cas9 protein was loaded on a gel as indicated and then detected by Western blot using GenCrispr Cas9 antibody (1:3000 dilution) and Goat Anti-Rabbit IgG (H&L) [HRP],pAb (1: 10000 dilution).
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit Monoclonal Antibody
WB
Unconjugated
40 µl/$295; 100 µl/$379
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunoprecipitation of SaCas9 from Hela cell transfected with PX458 (SaCas9(BB)-2A-GFP) by using  SaCas9 Antibody (26H10). Predicted band size: 124 kDa. Loading: Lane 1: Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) 50µg (Input). Lane 2: Protein A MagBeads (20µl) + Purified antibody (10µg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200µg). Lane 3: Protein A MagBeads (20µl) + Purified antibody (10µg) + Hela cell lysate (200µg). Lane 4: Protein A MagBeads (20µl) + Mouse IgG (10µg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200µg). Lane 5: Protein A MagBeads (20µl) + Mouse IgG (10µg) + Hela Cell lysate (200µg). Lane 6: Hela Cell lysate (50µg). Primary Antibody: SaCas9 Antibody (26H10) 1 µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Staphylococcus aureus Monoclonal (26H10) Antibody
Staphylococcus aureus
ELISA, IF, WB
Unconjugated
40 µg/$295
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot analysis of HEK293 transfected with various plasmids with SpCas9 Antibody (4A1). The different HEK293 with transfected with various plasmids indicate the minimum cross reaction of the antibody. Loading: Lane 1: 50µg HEK293 cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus). Lane 2: 50µg HEK293 cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles). Lane 3: 50µg HEK293 cell lysate transfected with FnCpf1(BB)-2A-GFP (A0Q7Q2, Francisella tularensis subsp. novicida (strain U112)). Lane 4: 50µg HEK293 cell lysate transfected with LbCpf1(BB)-2A-GFP ( Lachnospiraceae bacterium ND2006). Lane 5: 50µg HEK293 cell lysate transfected with AsCpf1(BB)-2A-GFP (U2UMQ6, Acidaminococcus sp. (strain BV3L6)). Lane 6: 50µg HEK293 cell lysate transfected with pSpCas9(BB)-2A-GFP (Streptococcus pyogenes serotype M1). Lane 7: 50µg HEK293 cell lysate (Non-transfected). Primary Antibody: Lane 1~7: SpCas9 Antibody (4A1) 1 µg/ml. Lane 1~7: Anti-a-tubulin mAb (mouse)(1F4) 0.5µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (4A1) Antibody
Streptococcus pyogenes
ELISA, IF, WB
Unconjugated
40 µg/$295
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot analysis of HEK293 transfected with various plasmids with SpCas9 Antibody (14B6). The different HEK293 with transfected with various plasmids indicate the minimum cross reaction of the antibody. Loading: Lane 1: 50µg HEK293 cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus). Lane 2: 50µg HEK293 cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles). Lane 3: 50µg HEK293 cell lysate transfected with FnCpf1(BB)-2A-GFP (Francisella tularensis subsp. novicida (strain U112)). Lane 4: 50µg HEK293 cell lysate transfected with LbCpf1(BB)-2A-GFP (Lachnospiraceae bacterium ND2006). Lane 5: 50µg HEK293 cell lysate transfected with AsCpf1(BB)-2A-GFP (Acidaminococcus sp. (strain BV3L6)). Lane 6: 50µg HEK293 cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1). Lane 7: 50µg HEK293 cell lysate (Non-transfected). Primary Antibody: Lane 1~7: SpCas9 Antibody (14B6) 1 µg/ml. Lane 1~7: Anti-a-tubulin mAb (mouse)(1F4) 0.5µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (14B6) Antibody
Streptococcus pyogenes
ELISA, IF, WB
Unconjugated
40 µg/$295
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunoprecipitation of SaCas9 from Hela cell transfected with PX458 (SaCas9(BB)-2A-GFP) by using SaCas9 Antibody (11C12). Predicted band size: 124 kDa. Loading: Lane 1: Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) 50µg (Input). Lane 2: Protein A MagBeads (20µl) + Purified antibody (10µg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200µg). Lane 3: Protein A MagBeads (20µl) + Purified antibody (10µg) + Hela cell lysate (200µg). Lane 4: Protein A MagBeads (20µl) + Mouse IgG (10µg) + Hela cell lysate transfected with PX458 (SaCas9(BB)-2A-GFP) (200µg). Lane 5: Protein A MagBeads (20µl) + Mouse IgG (10µg) + Hela Cell lysate (200µg). Lane 6: Hela Cell lysate (50µg). Primary Antibody: SaCas9 Antibody (11C12) 1 µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Staphylococcus aureus Monoclonal (11C12) Antibody
Staphylococcus aureus
ELISA, IF, WB
Unconjugated
40 µg/$295
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of HEK293 cells transfected with S. aureas Cas9 and labeled with Anti-CRISPR Cas9 (1:500, red).
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Staphylococcus aureus Monoclonal (C-term) (6F7) Antibody
Staphylococcus aureus
IF, IHC, WB
Unconjugated
100 µl/$469
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (C-Terminus) Antibody
Streptococcus pyogenes
ELISA, IF, WB
Unconjugated
25 µl/$304
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (C-Terminus) (Liquid) (Biotin) Antibody
Streptococcus pyogenes
ELISA, IF, WB
Biotin Conjugated, Liquid
25 µl/$304
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (C-Terminus) (Lyophilized) (HRP) Antibody
Streptococcus pyogenes
ELISA, IF, WB
HRP Conjugated, Lyophilized
100 µg/$544
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Immunofluorescence of rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1µg/mL. Secondary: Donkey anti-Rabbit at 1µg/mL, staining seen in red. Bottom images show GFP stain overlay.
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Rabbit anti-Streptococcus pyogenes Polyclonal (C-Terminus) (Liquid) (HRP) Antibody
Streptococcus pyogenes
ELISA, IF, WB
HRP Conjugated, Liquid
25 µl/$304
Select
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (aa1-1166) (OTI1C8) Antibody
Streptococcus pyogenes, Human
IP, WB
Unconjugated
100 µl/$434
Select
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (aa1-1166) (Carrier-free) (OTI6D9) Antibody
Streptococcus pyogenes
IP, WB
Unconjugated, Carrier-free
100 µg/$511
Select
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (aa1-1166) (OTI6D9) Antibody
Streptococcus pyogenes
IP, WB
Unconjugated
100 µl/$434
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (aa1-1166) (Carrier-free) (OTI5E5) Antibody
Streptococcus pyogenes, Human
IP, WB
Unconjugated, Carrier-free
100 µg/$511
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (aa1-1166) (OTI5E5) Antibody
Streptococcus pyogenes, Human
IP, WB
Unconjugated
100 µl/$434
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Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (aa1-1166) (Carrier-free) (OTI2B9) Antibody
Streptococcus pyogenes, Human
WB
Unconjugated, Carrier-free
100 µg/$511
Select
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (aa1-1166) (Carrier-free) (OTI1C8) Antibody
Streptococcus pyogenes, Human
IP
Unconjugated, Carrier-free
100 µg/$511
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