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| Catalog Number | Size | Price |
|---|---|---|
| LS-C829092-40 | 40 µg (0.5 mg/ml) | $295 |
![Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with two independent antibodies: SpCas9 Antibody (4A1) and SpCas9 Antibody (14B6). The correlated pattern indicates the high specificity of these two antibodies. Lane 1: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 2: 50 µg Untransfected HEK293 cell Lysate. Lane 3: 40 ng SpCas9 recombinant protein. Lane 4: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 5: 50 µg Untransfected HEK293 cell Lysate. Lane 6: 40 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~3: SpCas9 Antibody (4A1) 1 µg/ml. Lane 4~6: SpCas9 Antibody (14B6) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.](https://lsbio-7d62.kxcdn.com/image2/streptococcus-pyogenes-crispr-associated-endonuclease-cas9-csn1-antibody-clone-14b6-ls-c829092/622578_5684844.jpg)
![Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of recombinant Streptococcus pyogenes CRISPR/Cas9 protein with two independent antibodies: SpCas9 Antibody (4A1) and SpCas9 Antibody (14B6). The correlated pattern indicates the high specificity of these two antibodies. Lane 1: 50 ng SpCas9 recombinant protein. Lane 2: 25 ng SpCas9 recombinant protein. Lane 3: 10 ng SpCas9 recombinant protein. Lane 4: 5 ng SpCas9 recombinant protein. Lane 5: 50 ng SpCas9 recombinant protein. Lane 6: 25 ng SpCas9 recombinant protein. Lane 7: 10 ng SpCas9 recombinant protein. Lane 8: 5 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~4: SpCas9 Antibody (4A1) 1 µg/ml. Lane 5~8: SpCas9 Antibody (14B6) 1 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.](https://lsbio-7d62.kxcdn.com/image2/streptococcus-pyogenes-crispr-associated-endonuclease-cas9-csn1-antibody-clone-14b6-ls-c829092/622592_5684858.jpg)
![Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot analysis of HEK293 transfected with various plasmids with SpCas9 Antibody (14B6). The different HEK293 with transfected with various plasmids indicate the minimum cross reaction of the antibody. Loading: Lane 1: 50µg HEK293 cell lysate transfected with SaCas9(BB)-2A-GFP (J7RUA5, Staphylococcus aureus). Lane 2: 50µg HEK293 cell lysate transfected with StCas9(BB)-2A-GFP (G3ECR1, Streptococcus thermophiles). Lane 3: 50µg HEK293 cell lysate transfected with FnCpf1(BB)-2A-GFP (Francisella tularensis subsp. novicida (strain U112)). Lane 4: 50µg HEK293 cell lysate transfected with LbCpf1(BB)-2A-GFP (Lachnospiraceae bacterium ND2006). Lane 5: 50µg HEK293 cell lysate transfected with AsCpf1(BB)-2A-GFP (Acidaminococcus sp. (strain BV3L6)). Lane 6: 50µg HEK293 cell lysate transfected with pSpCas9(BB)-2A-GFP (PX458, Q99ZW2, Streptococcus pyogenes serotype M1). Lane 7: 50µg HEK293 cell lysate (Non-transfected). Primary Antibody: Lane 1~7: SpCas9 Antibody (14B6) 1 µg/ml. Lane 1~7: Anti-a-tubulin mAb (mouse)(1F4) 0.5µg/ml. Secondary antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.](https://lsbio-7d62.kxcdn.com/image2/streptococcus-pyogenes-crispr-associated-endonuclease-cas9-csn1-antibody-clone-14b6-ls-c829092/622566_5684832.jpg)
![Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody - Western Blot of HEK293 transfected with PX458 (pSpCas9(BB)-2A-GFP) or untransfected cell lysates with SpCas9 Antibody (14B6). The different concentration of antibodies indicates the high affinity and sensitivity of the antibody. Lane 1: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 2: 50 µg Untransfected HEK293 cell Lysate. Lane 3: 40 ng SpCas9 recombinant protein. Lane 4: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 5: 50 µg Untransfected HEK293 cell Lysate. Lane 6: 40 ng SpCas9 recombinant protein. Lane 7: 50 µg HEK293 transfected with PX458 cell Lysate. Lane 8: 50 µg Untransfected HEK293 cell Lysate. Lane 9: 40 ng SpCas9 recombinant protein. Primary Antibody: Lane 1~3: SpCas9 Antibody (14B6) 2 µg/ml. Lane 4~6: SpCas9 Antibody (14B6) 1 µg/ml. Lane 7~9: SpCas9 Antibody (14B6) 0.5 µg/ml. Secondary Antibody: Goat anti-Mouse IgG (H&L) [IRDye800] 0.125 µg/ml.](https://lsbio-7d62.kxcdn.com/image2/streptococcus-pyogenes-crispr-associated-endonuclease-cas9-csn1-antibody-clone-14b6-ls-c829092/622574_5684840.jpg)
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Monoclonal Mouse anti‑Streptococcus pyogenes Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (clone 14B6, IF, WB) LS‑C829092
Monoclonal Mouse anti‑Streptococcus pyogenes Streptococcus pyogenes CRISPR‑associated endonuclease Cas9/Csn1 Antibody (clone 14B6, IF, WB) LS‑C829092
Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (14B6) Antibody
Application:
IF, WB, ELISA
Reactivity:
Streptococcus pyogenes
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Mouse anti-Streptococcus pyogenes Monoclonal (14B6) Antibody
Application:
IF, WB, ELISA
Reactivity:
Streptococcus pyogenes
Format:
Unconjugated, Unmodified
Specifications
Description
Csn1 antibody LS-C829092 is an unconjugated mouse monoclonal antibody to streptococcus pyogenes Csn1 (Streptococcus pyogenes CRISPR-associated endonuclease Cas9). Validated for ELISA, IF and WB.
Target
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1
Host
Mouse
Reactivity
Streptococcus pyogenes
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
14B6
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant Streptococcus pyogenes CRISPR/Cas9
Specificity
The product is specific for Streptococcus pyogenes CRISPR/Cas9. This antibody binds with recombinant Streptococcus pyogenes CRISPR/Cas9 protein in ELISA and Endogenous overexpressed Streptococcus pyogenes CRISPR/Cas9 in immunofluorescence, western blot.
Applications
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- ELISA (0.05 - 1 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from PBS, pH 7.4, 0.02% Sodium Azide
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage
Store lyophilized at -20°C for up to 1 year. Store reconstituted at 2°C to 8°C for up to 3 weeks. Aliquot and store at -20°C or below for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Streptococcus pyogenes
Applications:
Western blot
Species:
Streptococcus pyogenes
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Streptococcus pyogenes
Applications:
Western blot
Species:
Streptococcus pyogenes
Applications:
Immunofluorescence, Western blot
Species:
Streptococcus pyogenes
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Streptococcus pyogenes
Applications:
Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/27/2024
Date Requested: 12/27/2024
