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PRKAG1 / AMPK Gamma 1

protein kinase, AMP-activated, gamma 1 non-catalytic subunit

AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gene Name: protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Family/Subfamily: Protein Kinase Non Catalytic , AMPK/SNF1
Synonyms: PRKAG1, AMPK gamma 1, AMPK gamma-1 chain, AMPK subunit gamma-1, AMPKG, AMPK gamma1
Target Sequences: NM_002733 NP_002724.1 P54619

Publications (2)

1
Hypothalamic CaMKK2 contributes to the regulation of energy balance. Anderson KA, Ribar TJ, Lin F, Noeldner PK, Green MF, Muehlbauer MJ, Witters LA, Kemp BE, Means AR. Cell metabolism. 2008 7:377-88. (WB) [PubMed:18460329]
2
Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins. Ouyang T, Niu G, Zhang Y, Liu X, Zhang X, Zhang S, Geng Y, Pang D, Ouyang H, Ren L. Viruses. 2019 June;11:E544. [Full Text Article] [PubMed:31212640] [PMC:PMC6630565] Related Antibodies: LS-C326839.

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Proteins (8)
Over-Expression Lysate (2)
Recombinant (6)
PRKAG1 / AMPK Gamma 1 (8)
No (8)
GST (1)
His (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (7)
293T Cells (1)
E. coli (2)
HEK 293 Cells (2)
Insect Cells (2)
Wheat Germ Extract (1)
Purified (4)
Low endotoxin level (2)
PRKAG1 / AMPK Gamma 1 Protein - 12.5% SDS-PAGE of human PRKAG1 stained with Coomassie Blue
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Wheat Germ Extract
GST
62.04 kDa
10 µg/$479; 25 µg/$670
PRKAG1 / AMPK Gamma 1 Protein - Recombinant Protein Kinase, AMP Activated Gamma 1 By SDS-PAGE
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E. coli
His-T7
30.6 kDa
10 µg/$281; 50 µg/$440; 100 µg/$702; 200 µg/$877; 1 mg/$1,983; 500 µg/$1,523; 5 mg/$3,067; 2 mg/$2,170
PRKAG1 / AMPK Gamma 1 Protein - Purified recombinant protein PRKAG1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
28.1 kDa
20 µg/$1,107
PRKAG1 / AMPK Gamma 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
37.4 kDa
20 µg/$150
PRKAG1 / AMPK Gamma 1 Protein
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E. coli
His
50 µg/$361; 250 µg/$739; 10 µg/$261
PRKAG1 / AMPK Gamma 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
37.4 kDa
100 µg/$494
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Insect Cells
None
Less than 1.0 EU/µg protein (determined by LAL method).
158 kDa
50 µg/$1,133
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Insect Cells
None
Less than 1.0 EU/µg protein (determined by LAL method).
160 kDa
50 µg/$1,133
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).