Target
PRKAG1 / AMPK Gamma 1
Synonyms
PRKAG1 | AMPK gamma 1 | AMPK gamma-1 chain | AMPK subunit gamma-1 | AMPKG | AMPK gamma1
Conjugations
Unconjugated
Predicted Molecular Weight
158 kDa
Expression System
Insect Cells
Purification
Greater than 90% by SDS-PAGE
Usage Summary
A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
sterile 50 mM Tris, pH 7.4, 1 mM EDTA, 200 mM NaCl, 0.5 mM PMSF, 1 mM DTT, 10% Glycerol, 1 mM GSH
Storage
Store at -20°C or below for up to 6 months. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKAG1 / AMPK Gamma 1