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PPP1R12A / MYPT1
protein phosphatase 1, regulatory subunit 12A
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
| Gene Name: | protein phosphatase 1, regulatory subunit 12A |
| Synonyms: | PPP1R12A, M130, MYPT1, MBS, Myosin binding subunit |
| Target Sequences: | NM_002480 NP_002471.1 O14974 |
Publications (2)
1
Physiological vs. pharmacological signalling to myosin phosphorylation in airway smooth muscle. Gao N, Tsai MH, Chang AN, He W, Chen CP, Zhu M, Kamm KE, Stull JT. The Journal of physiology. 2017 October;595:6231-6247. [Full Text Article]
[PubMed:28749013]
[PMC:PMC5621497]
Related Antibodies: LS-C117671.
2
Constitutive Phosphorylation of Cardiac Myosin Regulatory Light Chain in vivo. Battiprolu PK, Cowley PM, Chen G, Gerard RD, Pinto JR, Hill JA, Baker AJ, Kamm KE, Stull JT. The Journal of biological chemistry. Mar; [Full Text Article]
[PubMed:25733667]
Related Antibodies: LS-C117671.
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PPP1R12A / MYPT1
(57)
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(56)
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(6)
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(4)
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(4)
PPP1R12A / MYPT1 Goat anti-Human Polyclonal Antibody
Mouse, Dog, Bovine, Rat, Horse, Bat, Human, Monkey
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µg/$485
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (aa661-710) Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (aa800-850) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (pThr696) Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µl/$334; 100 µl/$397
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$440; 100 µg/$480
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (aa621-670) Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IP, WB
Unconjugated
20 µl/$275; 100 µl/$389
PPP1R12A / MYPT1 Rabbit anti-Human Monoclonal (pThr850) Antibody
Chicken, Human
WB
Unconjugated
100 µl/$720
Cell-Based Phosphorylation
96-Well Microplate
Mouse, Human, Rat
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
1 Plate/$668
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (pThr853) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (pThr853) Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (pThr853) Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µg/$305; 100 µg/$357
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (pThr850) Antibody
Chicken, Zebrafish, Horse, Rat, Rabbit, Mouse, Dog, Human, Monkey
WB
Unconjugated
200 µg/$1,054
Cell-Based
96-Well Microplate
Mouse, Human, Rat
Colorimetric - 450nm (TMB)
Adherent Cell Cultures
1 Plate/$534
HEK 293 Cells
Myc-DDK (Flag)
115.1 kDa
20 µg/$1,107
293T Cells
Myc-DDK (Flag)
117 kDa
20 µg/$215
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (aa1-40) Antibody
Mouse, Dog, Guinea pig, Sheep, Bovine, Rat, Pig, Horse, Bat, Human
IHC, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (630-710 aa) Antibody
Mouse, Rat, Human
ELISA, IF, IHC
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (pThr696) Antibody
Mouse, Rat, Human
ELISA, IF, IHC
Unconjugated
100 µg/$357
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$331
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (590-670 aa) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (p696) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100 µg/$357
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (590-670 aa) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
PPP1R12A / MYPT1 Rabbit anti-Human Polyclonal (p853) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100 µg/$357
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