Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers

Customer Service: (800) 227-6666 / (650) 697-3600

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-B3836-50	50 µg (0.5 mg/ml)	$485

PPP1R12A / MYPT1 Antibody - Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

PPP1R12A / MYPT1 Antibody - PPP1R12A / MYPT1 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

PPP1R12A / MYPT1 Antibody - PPP1R12A / MYPT1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

PPP1R12A / MYPT1 Antibody - PPP1R12A / MYPT1 antibody flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

1 of 4

2 of 4

3 of 4

4 of 4

IHC‑plus™ Polyclonal Goat anti‑Human PPP1R12A / MYPT1 Antibody (IHC, IF) LS‑B3836

Name: IHC-plus™ PPP1R12A / MYPT1 Polyclonal Antibody Goat anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-B3836-50
Price: 485.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

(4.0) Rating

Submit Review LSBio Guarantee

Note: This antibody replaces LS-C108241

Antibody:

PPP1R12A / MYPT1 Goat anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IF, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse

Format:

Unconjugated, Unmodified

$485

LS-B3836-50

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PPP1R12A / MYPT1 Goat anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IF, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse

Format:

Unconjugated, Unmodified

Specifications

Description

MYPT1 antibody LS-B3836 is an unconjugated goat polyclonal antibody to MYPT1 (PPP1R12A) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.

Target

Human PPP1R12A / MYPT1

Synonyms

PPP1R12A | M130 | MYPT1 | MBS | Myosin binding subunit

See All PPP1R12A / MYPT1 Antibodies

Host

Goat

Reactivity

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-REDEYKQKYSRTYD, from the C-Terminus of protein sequence according to NP_002471.1, NP_001231919.1, NP_001231921 .1, NP_001137358.1.

Specificity

Human PPP1R12A / MYPT1.

Applications

IHC
IHC - Paraffin (3.75 µg/ml)
Immunofluorescence (10 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:16000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B3836 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3836 was determined to be 3.75ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PPP1R12A / MYPT1

Uniprot: O14974 NCBI: NM_002480 NP_002471.1

Validation

Anti-PPP1R12A / MYPT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

See More About...

About LSBio Validation process