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KIF11 / EG5

kinesin family member 11

Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Gene Name: kinesin family member 11
Synonyms: KIF11, EG5, Kinesin family member 11, Kinesin-like protein 1, Kinesin-like 1, Kinesin-like protein KIF11, MCLMR, TRIP5, HKSP, KNSL1, TR-interacting protein 5, TRIP-5
Target Sequences: NM_004523 NP_004514.2 P52732

Publications (5)

1
Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Ferenz NP, Wadsworth P. Molecular biology of the cell. 2007 18:3993-4002. [PubMed:17671163] [PMC:PMC1995704]
2
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis. Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T. Circulation research. 2009 105:500-10. [PubMed:19644050]
3
Kinesin molecular motor Eg5 functions during polypeptide synthesis. Bartoli KM, Jakovljevic J, Woolford JL, Saunders WS. Molecular biology of the cell. 2011 22:3420-30. [PubMed:21795388] [PMC:PMC3172266] Related Antibodies: LS-C40939.
4
Dynamic reorganization of Eg5 in the mammalian spindle throughout mitosis requires dynein and TPX2. Gable A, Qiu M, Titus J, Balchand S, Ferenz NP, Ma N, Collins ES, Fagerstrom C, Ross JL, Yang G, Wadsworth P. Molecular biology of the cell. 2012 23:1254-66. (ICC, WB; Pig) [PubMed:22337772] [PMC:PMC3315814]
5
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Lawo S, Hasegan M, Gupta GD, Pelletier L. Nature cell biology. 2012 14:1148-58. (WB; Human) [PubMed:23086237]
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☰ Filters
Antibodies (37)
Proteins (4)
ELISA Kits (2)
Cell-Based (1)
Cell-Based Phosphorylation (1)
Over-Expression Lysate (2)
Primary (37)
Recombinant (2)
KIF11 / EG5 (43)
Human (39)
Mouse (7)
Rat (3)
Drosophila (1)
Pig (1)
IHC (20)
IHC-P (7)
WB (33)
Flo (7)
ELISA (18)
ICC (3)
IF (10)
IP (7)
Peptide-ELISA (3)
rabbit (31)
mouse (6)
Product Group
IHCPlus (3)
IgG (24)
IgG1 (2)
IgG1,k (1)
IgG2a (1)
monoclonal mc (6)
polyclonal pc (31)
10C7/Eg5 (1)
3A1 (1)
96-Well Microplate (2)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (29)
aa103-130 (7)
870-950 aa (2)
pThr926 (2)
C-Terminus (1)
Pro923 (1)
aa1006-C-term (1)
aa200-500 (1)
aa892-941 (1)
aa925-975 (1)
p927 (1)
No (42)
Yes (1)
Adherent Cell Cultures (2)
GST, N-terminus (1)
Myc-DDK (Flag) (3)
Human (3)
293T Cells (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
KIF11 / EG5 Antibody - Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Eg5 (Clone 10C7/Eg5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated.
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KIF11 / EG5 Mouse anti-Human Monoclonal (10C7/Eg5) Antibody
Mouse, Human
WB
Unconjugated
25 µg/$279; 100 µg/$359
KIF11 / EG5 Antibody - Anti-KIF11 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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KIF11 / EG5 Rabbit anti-Human Polyclonal Antibody
Drosophila, Pig, Mouse, Rat, Human
IHC, IHC-P
Unconjugated
0.1 ml/$460
KIF11 / EG5 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
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KIF11 / EG5 Mouse anti-Human Monoclonal Antibody
Human
ICC, IHC, IHC-P, IP, WB
Unconjugated
100 µl/$460
KIF11 / EG5 Antibody - Anti-KIF11 / EG5 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
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KIF11 / EG5 Mouse anti-Human Monoclonal Antibody
Human
ICC, IHC, IHC-P, WB
Unconjugated
100 µl/$485
KIF11 / EG5 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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KIF11 / EG5 Rabbit anti-Human Polyclonal (aa103-130) Antibody
Human
Flo, IHC, IHC-P, WB
Unconjugated
400 µl/$393
KIF11 / EG5 Antibody - Immunohistochemical analysis of EG5 (pT926) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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KIF11 / EG5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$315
KIF11 / EG5 Antibody - P-peptide - + Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using KIF11/Eg5 (Phospho-Thr926) antibody.
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KIF11 / EG5 Rabbit anti-Human Polyclonal (pThr926) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$357
KIF11 / EG5 Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using KIF11/Eg5 (Ab-927)antibody.
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KIF11 / EG5 Rabbit anti-Human Polyclonal (p927) Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$357
KIF11 / EG5 Antibody - Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using KIF11/Eg5 Antibody. The picture on the right is blocked with the synthesized peptide.
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KIF11 / EG5 Rabbit anti-Human Polyclonal (aa892-941) Antibody
Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
KIF11 / EG5 Antibody - Western blot of Eg5 antibody
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KIF11 / EG5 Rabbit anti-Human Polyclonal (870-950 aa) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
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KIF11 / EG5 Rabbit anti-Human Polyclonal (aa103-130) Antibody
Human
ELISA, Flo, IHC, WB
Unconjugated
200 µl/$734
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KIF11 / EG5 Rabbit anti-Human Polyclonal (aa103-130) (Biotin) Antibody
Human
ELISA, Flo, IHC, WB
Biotin Conjugated
200 µl/$952
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KIF11 / EG5 Rabbit anti-Human Polyclonal (aa103-130) (FITC) Antibody
Human
ELISA, Flo, IHC, WB
FITC Conjugated
200 µl/$952
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KIF11 / EG5 Rabbit anti-Human Polyclonal (aa103-130) (APC) Antibody
Human
ELISA, Flo, IHC, WB
APC Conjugated
200 µl/$952
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KIF11 / EG5 Rabbit anti-Human Polyclonal (aa103-130) (PE) Antibody
Human
ELISA, Flo, IHC, WB
PE Conjugated
200 µl/$952
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KIF11 / EG5 Rabbit anti-Human Polyclonal (aa103-130) (Azide-free) (HRP) Antibody
Human
ELISA, Flo, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
KIF11 / EG5 Antibody - IHC of Eg5 (P923) pAb in paraffin-embedded human lung carcinoma tissue.
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KIF11 / EG5 Rabbit anti-Human Polyclonal (Pro923) Antibody
Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$321; 100 µl/$403
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KIF11 / EG5 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IP, WB
Unconjugated
100 µg/$351
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KIF11 / EG5 Mouse anti-Human Monoclonal (aa200-500) Antibody
Mouse, Human
IF, IP, WB
Unconjugated
25 µg/$449; 100 µg/$603
KIF11 / EG5 Antibody - Western Blot of Rabbit Anti-Eg-5 antibody. Lane 1: E.coli cell lysate expressing histidine tagged protein. Lane 2: Mouse brain lysate. Lane 3: Recombinant truncated Eg-5. Lane 4: MW. Load: 35 µg per lane for cell lysate. 50 ng of recombinant protein. Primary antibody: Eg-5 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT.
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KIF11 / EG5 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µl/$567
KIF11 / EG5 Antibody - Immunofluorescent analysis of Hela cells using KIF11 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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KIF11 / EG5 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, WB
Unconjugated
50 µg/$294; 100 µg/$360
KIF11 / EG5 Antibody - Western Blot of rabbit anti-Eg-5 Antibody. Lane 1: E.coli cell lysate expressing histidine tagged protein. Lane 2: Mouse brain lysate. Lane 3: Recombinant truncated Eg-5. Lane 4: MW. Load: 35 µg per lane for cell lysate. 50 ng of recombinant protein. Primary antibody: Eg-5 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 120 kDa and 72 kDa for Eg5.
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KIF11 / EG5 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
25 µl/$304
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KIF11 / EG5 Rabbit anti-Human Polyclonal Antibody
Human
IF, IP, WB
Unconjugated
100 µl/$649
KIF11 / EG5 Antibody - Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).
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KIF11 / EG5 Mouse anti-Human Monoclonal Antibody
Human
ELISA, WB
Unconjugated
50 µg/$332; 100 µg/$404; 200 µg/$505
KIF11 / EG5 Antibody - Staining COLO205 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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KIF11 / EG5 Rabbit anti-Human Polyclonal Antibody
Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Viewing 1-25 of 43 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).