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Catalog Number Size Price
LS-C356147-100 100 µl (1 mg/ml) $315 
KIF11 / EG5 Antibody - Immunohistochemical analysis of EG5 (pT926) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
KIF11 / EG5 Antibody - Immunofluorescent analysis of EG5 (pT926) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
KIF11 / EG5 Antibody - Western blot analysis of EG5 (pT926) expression in Jurkat (A); HEK293T (B) whole cell lysates.
KIF11 / EG5 Antibody - Immunohistochemical analysis of EG5 (pT926) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
KIF11 / EG5 Antibody - Immunofluorescent analysis of EG5 (pT926) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
KIF11 / EG5 Antibody - Western blot analysis of EG5 (pT926) expression in Jurkat (A); HEK293T (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human KIF11 / EG5 Antibody (C‑Terminus, IHC, IF, WB) LS‑C356147

Polyclonal Rabbit anti‑Human KIF11 / EG5 Antibody (C‑Terminus, IHC, IF, WB) LS‑C356147

Antibody:
KIF11 / EG5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$315
LS-C356147-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
KIF11 / EG5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
EG5 antibody LS-C356147 is an unconjugated rabbit polyclonal antibody to human EG5 (KIF11) (C-Terminus). Validated for ICC, IF, IHC and WB.
Target
Human KIF11 / EG5
Synonyms
KIF11 | EG5 | Kinesin family member 11 | Kinesin-like protein 1 | Kinesin-like 1 | Kinesin-like protein KIF11 | MCLMR | TRIP5 | HKSP | KNSL1 | TR-interacting protein 5 | TRIP-5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human EG5 (pT926).
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of EG5 (pT926) protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KIF11 / EG5
P52732 NM_004523 NP_004514.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/25/2024