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ATG4D

autophagy related 4D, cysteine peptidase

Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability.

Gene Name: autophagy related 4D, cysteine peptidase
Synonyms: ATG4D, APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D, Cysteine protease ATG4D, Autophagin-4
Target Sequences: NM_032885 NP_116274.3 Q86TL0

☰ Filters
Antibodies (61)
Proteins (1)
Primary (61)
Recombinant (1)
ATG4D (62)
Human (61)
Mouse (24)
Rat (10)
Bovine (3)
Chimpanzee (3)
Gibbon (3)
Monkey (3)
Pig (3)
Rabbit (3)
IHC (16)
IHC-P (4)
WB (54)
ELISA (23)
ICC (5)
IF (29)
IP (1)
Peptide-ELISA (2)
rabbit (53)
mouse (7)
goat (1)
Product Group
IHCPlus (1)
Ig (1)
IgG (24)
IgG1,k (7)
monoclonal mc (7)
polyclonal pc (54)
222CT15.4.1 (6)
APC Conjugated (5)
Atto 390 Conjugated (2)
Atto 488 Conjugated (2)
Atto 594 Conjugated (2)
Azide-free (2)
Biotin Conjugated (7)
FITC Conjugated (7)
HRP Conjugated (5)
PE Conjugated (3)
PerCP Conjugated (2)
RPE Conjugated (2)
Unconjugated (24)
aa321-346 (7)
aa200-249 (4)
Internal (1)
N-Terminus (1)
aa 326-356 (1)
aa220-249 (1)
aa42-71 (1)
aa444-457 (1)
No (62)
GST (1)
Human (1)
Wheat Germ Extract (1)
Purified (1)
ATG4D Antibody - Anti-ATG4D antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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ATG4D Goat anti-Human Polyclonal (aa444-457) Antibody
Human
IHC, IHC-P, Peptide-ELISA
Unconjugated
50 µg/$485
ATG4D Antibody - Western blot analysis of extracts of various cells.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
ATG4D Antibody - ATG4D Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.
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ATG4D Rabbit anti-Human Polyclonal (aa42-71) Antibody
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
400 µl/$393
ATG4D Antibody - 1:100 staining mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
ATG4D Antibody - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 ug/ml.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
0.1 mg/$460
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ATG4D Mouse anti-Human Monoclonal (222CT15.4.1) Antibody
Human
ELISA, IF, WB
Unconjugated
200 µl/$734
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ATG4D Mouse anti-Human Monoclonal (Biotin) (222CT15.4.1) Antibody
Human
ELISA, ICC, IF, WB
Biotin Conjugated
200 µl/$952
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ATG4D Mouse anti-Human Monoclonal (FITC) (222CT15.4.1) Antibody
Human
ELISA, ICC, IF, WB
FITC Conjugated
200 µl/$952
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ATG4D Mouse anti-Human Monoclonal (APC) (222CT15.4.1) Antibody
Human
ELISA, ICC, IF, WB
APC Conjugated
200 µl/$952
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ATG4D Mouse anti-Human Monoclonal (Azide-free) (HRP) (222CT15.4.1) Antibody
Human
ELISA, ICC, IF, WB
HRP Conjugated, Azide-free
200 µl/$952
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ATG4D Mouse anti-Human Monoclonal (PE) (222CT15.4.1) Antibody
Human
ELISA, ICC, IF, WB
PE Conjugated
200 µl/$952
ATG4D Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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ATG4D Rabbit anti-Human Polyclonal (aa220-249) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
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ATG4D Rabbit anti-Human Polyclonal (Internal) Antibody
Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ATG4D Antibody - Immunofluorescence of U251 cells, using ATG4D Antibody. U251 cells(right) were treated with Chloroquine (50 mu M,16h). Antibody was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
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ATG4D Mouse anti-Human Monoclonal Antibody
Human
IF, WB
Unconjugated
400 µl/$393
ATG4D Antibody - Immunohistochemical analysis of ATG4D staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IP, WB
Unconjugated
30 µl/$255; 100 µl/$299; 200 µl/$351
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ATG4D Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
100 µg/$854
ATG4D Antibody - Immunohistochemistry of paraffin-embedded Human liver cancer using ATG4D Polyclonal Antibody at dilution of 1:40.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
ATG4D Antibody - Immunohistochemistry of paraffin-embedded Human brain using ATG4D Polyclonal Antibody at dilution of 1:40.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
ATG4D Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4D Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4D Antibody. (D) Composite.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Human
IF, WB
Unconjugated
100 µg/$442
ATG4D Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4D Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4D Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4D Antibody (D) Composite.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, WB
Unconjugated
100 µg/$442
ATG4D Antibody - Detection of Atg4D in formaldehyde-fixed C2C12 cells.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Human
IF, WB
Unconjugated
100 µg/$537
ATG4D Antibody - Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with APG4D Antibody (S341) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ATG4D Rabbit anti-Human Polyclonal (aa 326-356) Antibody
Human
ELISA, IHC-P, WB
Unconjugated
400 µl/$393
Select
ATG4D Rabbit anti-Human Polyclonal (aa321-346) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
ATG4D Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$341
ATG4D Antibody - Western blot analysis of extracts from K562 cells, using ATG4D antibody.
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ATG4D Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$357
Viewing 1-25 of 62 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).