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Catalog Number Size Price
LS-B6488-50 50 µg (0.5 mg/ml) $485 
ATG4D Antibody - Anti-ATG4D antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

IHC‑plus™ Polyclonal Goat anti‑Human ATG4D Antibody (aa444‑457, IHC) LS‑B6488

IHC‑plus™ Polyclonal Goat anti‑Human ATG4D Antibody (aa444‑457, IHC) LS‑B6488

Note: This antibody replaces LS-C112790
Antibody:
ATG4D Goat anti-Human Polyclonal (aa444-457) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B6488-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ATG4D Goat anti-Human Polyclonal (aa444-457) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ATG4D antibody LS-B6488 is an unconjugated goat polyclonal antibody to human ATG4D (aa444-457). Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human ATG4D
Synonyms
ATG4D | APG4-D | APG4D | AUTL4 | APG4 autophagy 4 homolog D | Cysteine protease ATG4D | Autophagin-4
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence CSQLAQPTLRLPRT, from the internal region (near C-Terminus) of the protein sequence according to NP_116274.3.
Epitope
aa444-457
Specificity
Human ATG4D.
Applications
  • IHC
  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATG4D
Q86TL0 NM_032885 NP_116274.3

Validation

ATG4D Antibody - Anti-ATG4D antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-ATG4D antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-ATG4D antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

LSBio Ratings

IHC-plus™ ATG4D Antibody (aa444-457) for IHC LS-B6488 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024