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Vector Laboratories, Inc.
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ACTN3

actinin, alpha 3

ACTN3 is a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Gene Name: actinin, alpha 3
Synonyms: ACTN3, Actinin, alpha 3, Alpha-actinin skeletal muscle, Alpha-actinin-3, F-actin cross-linking protein
Target Sequences: NM_001104 NP_001095.2 Q08043

☰ Filters
Products
Antibodies (38)
Primary (38)
ACTN3 (38)
Human (38)
Mouse (13)
Rat (12)
Bovine (4)
Dog (3)
Guinea pig (3)
Hamster (3)
Horse (3)
Monkey (3)
Pig (3)
Rabbit (4)
IHC (13)
IHC-P (9)
WB (32)
Flo (3)
ELISA (13)
ICC (2)
IF (6)
Peptide-ELISA (1)
rabbit (37)
mouse (1)
Product Group
IHCPlus (3)
IgG (20)
IgG1 (1)
monoclonal mc (1)
polyclonal pc (37)
APC (1)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (4)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
DY488 Conjugated (1)
DY550 Conjugated (1)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (2)
Unconjugated (18)
aa162-416 (8)
aa409-437 (7)
aa180-229 (4)
10-90 aa, N-terminal (1)
Glu25 (1)
N-Terminus (1)
aa1-50 (1)
No (38)
ACTN3 Antibody - Anti-ACTN3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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ACTN3 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$460
ACTN3 Antibody - Anti-ACTN3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ACTN3 Rabbit anti-Human Polyclonal (aa180-229) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
ACTN3 Antibody - Anti-ACTN3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ACTN3 Rabbit anti-Human Polyclonal (aa180-229) (HRP) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
ACTN3 Antibody - Anti-ACTN3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ACTN3 Rabbit anti-Human Polyclonal (aa180-229) (Biotin) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
ACTN3 Antibody - Anti-ACTN3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ACTN3 Rabbit anti-Human Polyclonal (aa180-229) (FITC) Antibody
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
ACTN3 Antibody - ACTN3 was detected in paraffin-embedded sections of mouse skeletal muscle tissues using rabbit anti- ACTN3 Antigen Affinity purified polyclonal antibody
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ACTN3 Rabbit anti-Human Polyclonal Antibody
Human
Flo, ICC, IHC, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
ACTN3 Antibody - Anti-ACTN3 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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ACTN3 Rabbit anti-Human Polyclonal (aa409-437) Antibody
Human
IHC, IHC-P, WB
Unconjugated
200 µl/$460
ACTN3 Antibody - Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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ACTN3 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Rabbit, Mouse, Bovine, Rat, Human
ICC, IF, WB
Unconjugated
100 µl/$299
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ACTN3 Rabbit anti-Human Polyclonal (aa409-437) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
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ACTN3 Rabbit anti-Human Polyclonal (aa409-437) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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ACTN3 Rabbit anti-Human Polyclonal (aa409-437) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
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ACTN3 Rabbit anti-Human Polyclonal (aa409-437) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
Select
ACTN3 Rabbit anti-Human Polyclonal (aa409-437) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
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ACTN3 Rabbit anti-Human Polyclonal (aa409-437) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
ACTN3 Antibody - Western blot of Actinin-alpha3 antibody
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ACTN3 Rabbit anti-Human Polyclonal (10-90 aa, N-terminal) Antibody
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ACTN3 Antibody - Immunofluorescence staining of Hela cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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ACTN3 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF
Unconjugated
50 µg/$294; 100 µg/$360
ACTN3 Antibody - Western blot of Actinin-3 (E25) pAb in extracts from PC12 cells.
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ACTN3 Rabbit anti-Human Polyclonal (Glu25) Antibody
Mouse, Rat, Human
IF, WB
Unconjugated
50 µl/$321; 100 µl/$403
ACTN3 Antibody - Western blot analysis of extracts of various cell lines, using ACTN3 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 5s.
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ACTN3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, WB
Unconjugated
20 µl/$275; 100 µl/$389
ACTN3 Antibody - IHC testing of FFPE human lung cancer tissue with ACTN3 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
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ACTN3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC-P, WB
Unconjugated
100 µg/$575
ACTN3 Antibody - Immunofluorescence analysis of HeLa cells using ACTN3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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ACTN3 Rabbit anti-Human Polyclonal Antibody
Human
IF, WB
Unconjugated
20 µl/$275; 100 µl/$389
ACTN3 Antibody - Western blot analysis of Human fetal muscle tissue and HeLa cell, using ACTN3 Polyclonal Antibody at dilution of 1:400.
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ACTN3 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
ACTN3 Antibody - Western blot analysis of extracts from HT-29 cells, using ACTN3 antibody.
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ACTN3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
ACTN3 Antibody - IHC analysis of ACTN3 using anti-ACTN3 antibody. ACTN3 was detected in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ACTN3 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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ACTN3 Mouse anti-Human Monoclonal Antibody
Mouse, Rat, Human
IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
Select
ACTN3 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
ACTN3 Antibody - Western blot of recombinant ACTN3.
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ACTN3 Rabbit anti-Human Polyclonal (aa162-416) Antibody
Human
WB
Unconjugated
100 µl/$303; 200 µl/$343; 500 µl/$476; 1 ml/$543; 2 ml/$715; 5 ml/$1,182
Viewing 1-25 of 38 product results


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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).