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Catalog Number Size Price
LS-C747869-20 20 µl $275 
LS-C747869-100 100 µl $389 
ACTN3 Antibody - Immunofluorescence analysis of HeLa cells using ACTN3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ACTN3 Antibody - Western blot analysis of extracts of various cell lines, using ACTN3 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
ACTN3 Antibody - Immunofluorescence analysis of HeLa cells using ACTN3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ACTN3 Antibody - Western blot analysis of extracts of various cell lines, using ACTN3 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
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Polyclonal Rabbit anti‑Human ACTN3 Antibody (IF, WB) LS‑C747869

Polyclonal Rabbit anti‑Human ACTN3 Antibody (IF, WB) LS‑C747869

Antibody:
ACTN3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C747869-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ACTN3 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ACTN3 antibody LS-C747869 is an unconjugated rabbit polyclonal antibody to human ACTN3. Validated for IF and WB.
Target
Human ACTN3
Synonyms
ACTN3 | Actinin, alpha 3 | Alpha-actinin skeletal muscle | Alpha-actinin-3 | F-actin cross-linking protein
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 580-630 of human ACTN3 (NP_001095.2). GAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSCDQ
Applications
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 103kDa, while the observed MW by Western blot was 103kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTN3
Q08043 NM_001104 NP_001095.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024