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| Catalog Number | Size | Price |
|---|---|---|
| LS-G3864-10 | 10 µg | $330 |
| LS-G3864-50 | 50 µg | $456 |
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Rat GRN / Granulin Protein (Recombinant DDK (Flag)) (aa1-602) - LS-G3864
Rat GRN / Granulin Protein (Recombinant DDK (Flag)) (aa1-602) - LS-G3864
Description:
GRN / Granulin Protein LS-G3864 is a Recombinant Rat GRN / Granulin produced in HEK 293 Cells aa 1-602 with DDK (Flag) tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
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(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
GRN / Granulin Protein LS-G3864 is a Recombinant Rat GRN / Granulin produced in HEK 293 Cells aa 1-602 with DDK (Flag) tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
GRN / Granulin
Synonyms
GRN | Acrogranin | CLN11 | Epithelin precursor | GEP | gp88 | Granulin | Granulin-epithelin | Granulins | PC cell-derived growth factor | PCDGF | PEPI | PGRN | Proepithelin | Progranulin
Species
Rat
Modifications
Unmodified
Conjugations
Unconjugated
Tag
DDK (Flag)
Region
aa 1-602
Predicted Molecular Weight
~65kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Not Tested
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Storage
Store lyophilized at -20°C for up to 1 year. Once reconstituted store at 4°C for immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GRN / Granulin
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Images
Functional Assay
Deglycosylation of rat progranulin. To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100 degrees C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two-fold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37 degrees C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.
Functional Assay
NH2-terminal sequence analysis.
Functional Assay
Deglycosylation of rat progranulin. To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100 degrees C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two-fold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37 degrees C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.
Functional Assay
NH2-terminal sequence analysis.
Functional Assay
Deglycosylation of rat progranulin. To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100 degrees C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two-fold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37 degrees C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.
Functional Assay
NH2-terminal sequence analysis.
Functional Assay
Deglycosylation of rat progranulin. To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100 degrees C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two-fold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37 degrees C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.
Functional Assay
NH2-terminal sequence analysis.
Popular GRN / Granulin Proteins
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
Date Requested: 11/27/2024
